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大豆gma-miR160o启动子的克隆及植物表达载体构建

DOI: 10.7668/hbnxb.2015.01.004, PP. 19-23

Keywords: 大豆,gma-miR160o,启动子,植物表达载体

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Abstract:

为了研究miRNA表达的调控机制,以大豆Williams82基因组DNA为材料,根据预测的gma-miR160o启动子序列设计引物,采用PCR方法克隆gma-miR160o上游一段长度为1910bp的启动子序列。采用PlantCARE和PLACE启动子在线预测工具分析表明,gma-miR160o启动子序列具有TATA-box、CAAT-box基本的顺式作用元件和一些参与非生物胁迫、光和植物激素应答相关的顺式作用元件。将gma-miR160o启动子替代pBI121载体中的CaMV35S启动子,构建了与GUS融合的启动子表达载体。

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