全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

牡丹PsAP1基因的表达分析及功能鉴定

DOI: 10.7668/hbnxb.2015.01.014, PP. 84-89

Keywords: PsAP1,表达分析,异源表达,开花时间

Full-Text   Cite this paper   Add to My Lib

Abstract:

为进一步探讨MADS-box基因家族在牡丹花器官发育和开花时间调控中的功能提供理论依据,为培育早花品种提供候选基因。以RACE扩增获得PsAP1基因的全长cDNA为基础,分析了其表达模式和功能,以牡丹课题组利用454测序得到的类AP1基因的部分cDNA序列为基础,利用RACE扩增方法获得1130bp牡丹PsAP1基因全长cDNA,同源性分析表明,牡丹PsAP1与葡萄VvAP1的相似性最高,为80.4%。实时定量PCR分析了其初花期的表达模式,结果表明,PsAP1基因在萼片中转录量最高,其次为花瓣,在雄蕊中表达量最低。将PsAP1基因在拟南芥中异源表达,分析鉴定了3个转基因株系的表型,结果发现异源表达PsAP1拟南芥开花时间较野生型提前了约3d。

References

[1]  Komeda Y.Genetic regulation of time to flower in Arabidopsis thaliana[J].Annual Review of Plant Biology,2004,55:521-535.
[2]  Mouradov A,Cremer F,Coupland G.Control of flowering time interacting pathways as a basis for diversity[J].The Plant Cell Online,2002,14(suppl 1):S111-S130.
[3]  Putterill J,Laurie R,Macknight R.It''s time to flower:the genetic control of flowering time[J].BioEssays,2004,26(4):363-373.
[4]  Farrona S,Coupland G,Turck F.The impact of chromatin regulation on the floral transition [C].Seminars in cell & developmental biology,2008,19(6):560-573.
[5]  Sablowski R.Flowering and determinacy in Arabidopsis[J].Journal of Experimental Botany,2007,58(5):899-907.
[6]  Becker A,Theissen G.The major clades of MADS-box genes and their role in the development and evolution of flowering plants[J].Molecular Phylogenetics and Evolution,2003,29(3):464-489.
[7]  Mandel M A,Gustafson-Brown C,Savidge B,et al.Molecular characterization of the Arabidopsis floral homeotic gene APETALA1 [J].Nature,1992,360:273-277.
[8]  Weigel D,Nilsson O.A developmental Switch sufficient for flower initiation in diverse plants[J].Nature,1995,377(6549):495-500.
[9]  Sarah J,Gustafson-Brown C,Pinyopich A,et al.Interactions among APETALA1,LEAFY,and TERMINAL FLOWER1 specify meristem fate[J].The Plant Cell Online,1999,11(6):1007-1018.
[10]  吕晋慧,吴月亮,孙 磊,等. AP1 基因转化地被菊品种玉人面的研究[J].林业科学,2007,43(9):128-132.
[11]  Chi Y J,Huang F,Liu H C,et al.An APETALA1-like gene of soybean regulates flowering time and specifies floral organs[J].Journal of Plant Physiology,2011,168(18):2251-2259.
[12]  Gai S P,Zhang Y X,Mu P,et al.Transcriptome analysis of tree peony during chilling requirement fulfillment:Assembling,annotation and markers discovering[J].Gene,2012,497(2):256-262.
[13]  Livak K J,Schmittgen T D.Analysis of relative gene expression data using Real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25:402-408.
[14]  Clough S J,Bent A F.Floral Dip:a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana[J].The Plant Journal,1998,16(6):735-743.
[15]  Calonje M,Cubas P,Martinez-Zapater J M,et al.Floral meristem identity genes are expressed during tendril development in grapevine[J].Plant Physiology,2004,135(3):1491-1501.
[16]  Kotoda N,Wada M,Komori S, et al. Expression pattern of homologues of floral meristem identity genes LFY and AP1 during flower development in Apple[J].Journal of the American Society for Horticultural Science,2000,125(4):398-403.
[17]  Pillitteri L J,Lovatt C J,Walling L L.Isolation and characterization of LEAFY and APETALA1 homologues from Citrus sinensis L.Osbeck Washington''[J].Journal of the American Society for Horticultural Science,2004,129(6):846-856.
[18]  Liu Y,Kong J,Li T,et al.Isolation and characterization of an APETALA1-like gene from pear(Pyrus pyrifolia) [J].Plant Molecular Biology Reporter,2013,31(4):1031-1039.
[19]  Li C,Xie H,Zhang L,et al.Molecular characterization of the PpMADS1 gene from peach[J].Tree Genetics & Genomes,2012,8(4):831-840.
[20]  孟玉平,曹秋芬,孙海峰.枣树花分生组织特异基因 ZjAP1 的克隆与表达分析[J].山西农业科学,2010,38(2):6-11.
[21]  王 娟,田建保,杜俊杰,等.Y系山定子 AP1 同源基因的克隆及序列分析[J].华北农学报,2014,29(5):66-70.
[22]  Kim S,Koh J,Ma H,et al.Sequence and expression studies of A-,B-,and E-class MADS-box homologues in Eupomatia (Eupomatiaceae):Support for the bracteate origin of the calyptra[J].International Journal of Plant Sciences,2005,166(2):185-198.
[23]  Fornara F,Parenicova L,Falasca G,et al.Functional characterization of OsMADS18,a member of the AP1/SQUA subfamily of MADS box genes[J].Plant Physiology,2004,135(4):2207-2219.
[24]  罗 聪,何新华,陈 虎,等.芒果 AP1 同源基因的克隆及其生物信息学分析[J].基因组学与应用生物学,2009,28(5):851-858.
[25]  刘志雄,王 莹,吕小蒙,等.日本晚樱花器官特征基因 ClAP1 的克隆与表达分析[J].园艺学报,2010,37(4):649-654.
[26]  Zhang L,Xu Y,Ma R.Molecular cloning,identification,and chromosomal localization of two MADS box genes in peach (Prunus persica) [J].Journal of Genetics and Genomics,2008,35:365-372.
[27]  张素芝,左建儒.拟南芥开花时间调控的研究进展[J].生物化学与生物物理进展,2006,9(3):301-309.
[28]  Srikanth A,Schmid M.Regulation of flowering time:all roads lead to Rome[J].Cellular and Molecular Life Sciences,2011,68(12):2013-2037.
[29]  Liu C,Xi W Y,Shen L S,et al.Regulation of floral patterning by flowering time genes[J].Developmental Cell,2009,16(5):711-722.
[30]  Gregis V,Sessa A,Dorca-Fornell C,et al.The arabidopsis floral meristem identity genes AP1,AGL24 and SVP directly repress class B and C floral homeotic genes[J].The Plant Journal,2009,60(4):626-637.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133