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番茄NAC转录因子SlNAC80的克隆及表达分析

DOI: 10.7668/hbnxb.2015.01.013, PP. 77-83

Keywords: 番茄,NAC转录因子,基因克隆,表达分析,非生物胁迫

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Abstract:

为进一步明确该基因的分子特征及表达特性,通过RT-PCR从番茄中克隆了低温响应NAC转录因子SlNAC80,该基因开放阅读框1023bp,编码340个氨基酸。SlNAC80蛋白相对分子量为38.19kDa,等电点为5.27,N-端具有典型的NAC保守结构域,包含A、B、C、D、E5个亚结构域。实时荧光定量PCR(qPCR)分析表明,SlNAC80在番茄各组织中均有表达,以在绿果和衰老叶中的表达量最高,在红熟果中的表达量最低。对启动子区序列进行预测分析发现,SlNAC80启动子区含有许多响应激素(ABA、GA、SA及ETH)和逆境(低温、脱水及盐胁迫)的顺式作用元件,如ERELEE4、GARE1OSREP1、LTRE1HVBLT49、GT1GMSCAM4、MYB1AT、MYCATERD1、MYCATRD22、WBOXNTERF3及WRKY71OS等。qPCR分析结果也证实该基因的表达受低温、干旱、高盐、甲基紫精、ABA及乙烯处理诱导。这些结果表明,SlNAC80可能在番茄非生物逆境应答中发挥重要调控作用。

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