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棉花抗枯萎病相关转录因子基因GhERFB101的克隆与表达分析

DOI: 10.7668/hbnxb.2015.01.011, PP. 66-72

Keywords: 棉花,ERF-B1转录因子,枯萎病菌,基因克隆,表达分析

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Abstract:

为了探究ERF转录因子家族与棉花枯萎病抗性之间的关系,也为海岛抗枯萎病品种的选育工作提供新的基因资源,从Solexa高通量测序技术建立的棉花基因表达谱中筛选探针序列,通过电子克隆结合RT-PCR技术从高抗枯萎病的棉花品种中棉所12中克隆到一个新的ERF-B1亚组转录因子基因,命名为GhERFB101(GenBank:KF850521)。序列分析表明,该基因开放阅读框738bp,编码245个氨基酸,含有一个保守的AP2/ERF结构域,在进化上与拟南芥AtERF11的亲缘关系最近。实时荧光定量PCR分析显示,枯萎病菌诱导后,GhERFB101基因在抗病品种根中的表达量呈现下降趋势;而在感病品种中,该基因呈上调表达,随着病菌处理后时间延长,其表达量呈现先增加后降低的变化趋势,在病菌处理后12h表达量达到最大,在48h下降到最低。乙烯和茉莉酸诱导后,该基因表达量均呈现明显的先增加后降低的变化趋势,在乙烯处理后2h基因的表达量达到最大;茉莉酸则在处理后1h基因的表达量达到最大;而水杨酸诱导后基因的变化幅度不大;推测该基因可能通过茉莉酸、乙烯信号传导途径参与对枯萎病菌的防御反应。

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