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青花菜BoPGIP1基因的原核表达及其重组大肠杆菌的抗逆性分析

DOI: 10.7668/hbnxb.2015.01.005, PP. 24-28

Keywords: 青花菜,BoPGIP1,原核表达,抗性分析

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Abstract:

为进一步验证青花菜BoPGIPl基因的功能,通过RT-PCR方法克隆得到青花菜BoPGIP1基因完整的编码区序列,将其克隆到原核表达载体pET28a(+)上,转化大肠杆菌BL21(DE3),获得高效表达BoPGIP1基因的重组大肠杆菌BL21(pET28a-BoPGIP1),重组菌经IPTG诱导表达以及SDS-PAGE电泳检测,结果表明,在37kDa处有一条特异表达的蛋白质条带,与预期的目的产物大小一致;同时对重组大肠杆菌BL21(pET28a-BoPGIP1)的抗逆性进行分析,结果表明,BL21(pET28a-BoPGIP1)对NaCl(0.4mol/L)、NaHCO3(0.2mol/L)和PEG6000(20%)的抗性明显高于对照菌株BL21(pET28a),该抗性的证明为BoPGIP1基因在植物抗逆基因工程中的应用提供了理论依据。

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