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湖泊科学  2013 

长江中下游5个湖泊黄颡鱼(Pelteobagrusfulvidraco)种群线粒体细胞色素b基因的遗传变异分析

DOI: 10.18307/2013.0219

Keywords: 黄颡鱼,线粒体DNA,细胞色素b基因,遗传变异,长江中下游

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Abstract:

对长江中下游5个湖泊的黄颡鱼种群的线粒体DNA细胞色素b基因进行了PCR扩增、测序,获得了955bp的序列.分析显示,cytb序列中A+T含量略高于G+C含量,共检测到54个变异位点,60个样本得到37个单倍型,平均单倍型多样性为0.945±0.018,核苷酸多样性为0.00419±0.00043,遗传多样性表现中等.太湖种群与滆湖种群间的遗传距离最远为0.00651,鄱阳湖种群和巢湖种群之间遗传距离最近为0.00375.分子方差分析(AMOVA)表明,群体间遗传分化系数Fst为0.0684,几乎所有变异都来自群体内,群体间遗传分化极小.cytb序列构建UPGMA系统进化树显示,5个种群没有分化成不同的分枝谱系,种群间存在广泛的基因交流.

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