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湖泊科学  2013 

营养盐对微囊藻细胞组分及多糖组成的影响

DOI: 10.18307/2013.0215

Keywords: 微囊藻,多糖,蛋白质,RNA

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Abstract:

胞外多糖是微囊藻形成群体的物质基础,营养盐是否会通过影响微囊藻多糖的含量进而影响群体的形成还不清楚.在室内培养实验中,设置不同N、P浓度的培养液并测定对数期胞内的细胞组分以及3种形态的多糖含量,从而探讨N、P对细胞中多糖的影响规律.实验结果表明:RNA在细胞中的含量相对恒定(约2.0pg/cell).N对单细胞的蛋白质和多糖都产生了显著影响:N浓度的升高显著促进蛋白质的合成,细胞中总糖的含量降低.P浓度的升高同样造成细胞中多糖总量减少,但其对蛋白质影响不大(在4.6pg/cell左右波动).N浓度的增加还促进多糖向胞外分泌并溶解于培养基中.在高生长率下,由于细胞中RNA、蛋白质的合成速度加快,多糖的合成较慢导致胞外固着性多糖减少,这可能是室内培养难以得到群体的原因之一.

References

[1]  Reynolds CS,Jaworski GHM,Cmiech HA et al. On the annual cycle of the blue-green alga Microcystis aeruginosa Kütz. Emend. Elenkin. Philosophical Transactions of the Royal Society of London Series B,Biological Sciences,1981,293 (1068): 419-477.
[2]  Bolch CJS,Blackburn SI. Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kütz. Journal of Applied Phycology,1996,8: 5-13.
[3]  Wu XD,Kong FX. Effects of light and wind speed on the vertical distribution of Microcystis aeruginosa colonies of different sizes during a summer bloom. International Review of Hydrobiology,2009,94(3): 258-266.
[4]  Yang Z,Kong FX,Yang Z et al. Benefits and costs of the grazer-induced colony formation in Microcystis aeruginosa. Annales de Limnologie-International Journal of Limnology,2009,45: 203-208.
[5]  Kessel M,Eloff JN. The ultrastructure and development of the colonial sheath of Microcystis marginata. Archives of Microbiology, 1975,106(3): 209-214.
[6]  Plude JL,Parker DL,Schommer OJ et al. Chemical characterization of polysaccharide from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40. Applied and Environmental Microbiology,1991,57(6): 1696-1700.
[7]  De Philippis R,Vincenzini M. Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiology Reviews,1998,22(3): 151-175.
[8]  Yang Z,Kong FX,Shi XL et al. Changes in the morphology and polysaccharide content of Microcystis aeruginosa (Cyanobacteria) during flagellate grazing. Journal of Phycology,2008,44(3): 716-720.
[9]  Liu Y,Wang W,Zhang M et al. PSII-efficiency,polysaccharide production,and phenotypic plasticity of Scenedesmus obliquus in response to changes in metabolic carbon flux. Biochemical Systematics and Ecology,2010,38: 292-299.
[10]  张晓峰,孔繁翔,曹焕生等. 太湖梅梁湾水华蓝藻复苏过程的研究. 应用生态学报,2 005,1 6(7): 1346-1350.
[11]  Piorreck M,Baasch KH,Pohl P. Biomass production,total protein,chlorophylls,lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry,1984,23(2): 207-216.
[12]  Downing TC,Sember CS,Gehringer MM et al. Medium N: P ratios and specific growth rate comodulate microcystin and protein content in Microcystis aeruginosa PCC7806 and M. aeruginosa UV027. Microbial Ecology,2005,49 (3): 468-473.
[13]  Vézie C,Rapala J,Vaitomaa J et al. Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microcystis strains and on intracellular microcystin concentrations. Microbial Ecology,2002,43(4): 443-454.
[14]  施军琼,马剑敏,吴忠兴. 环境因子对铜绿微囊藻7820 胞外多糖的影响. 河南师范大学学报, 2008,3 6(5): 120-123.
[15]  雷腊梅,宋立荣,欧丹云等. 营养条件对水华蓝藻铜绿微囊藻的胞外多糖产生的影响. 中山大学学报,2 007,4 6(3): 84-87.
[16]  Burkert U,Hyenstrand P,Drakare S et al. Effects of the mixotrophic flagellate Ochromonas sp. on colony formation in Microcystis aeruginosa. Aquatic Ecology,2001,35(1): 9-17.
[17]  杨州,李佳佳. 非生物因素对藻类胞外多聚糖含量影响. 应用生态学报,2008, 19(1): 198-202.
[18]  De Philippis R,Margheri MC,Pelosi E et al. Exopolysaccharide production by a unicellular cyanobacterium isolated from a hypersaline habitat. Journal of Applied Phycology,1993,5: 387-394.
[19]  曹焕生,孔繁翔,谭啸等. 太湖水华蓝藻底泥中复苏和水柱中生长的比较. 湖泊科学, 2006, 18(6): 585-589.

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