全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
湖泊科学  2013 

沉水植物生态修复对西湖细菌多样性及群落结构的影响

DOI: 10.18307/2013.0203

Keywords: 沉水植物,生态修复,细菌多样性,变性梯度凝胶电泳,克隆文库,西湖

Full-Text   Cite this paper   Add to My Lib

Abstract:

沉水植物的重建是湖泊富营养化修复的关键措施之一.本研究利用在杭州西湖进行的沉水植物生态修复工程,应用基于聚合酶链式反应的变性梯度凝胶电泳(PCR-DGGE)及分子克隆等手段,研究种植菹草和苦草对水体中细菌多样性及群落结构的影响.未进行沉水植物生态修复的湖区细菌以Bacteroidetes(42.9%)、Betaproteobacteria(30.8%)和Verru-comicrobia(14.3%)为主.而种植菹草和苦草后,Bacteroidetes所占的比例分别减少到12.7%和5.3%;Betaproteobacteria则分别提高到52.4%和59.5%;Alphaproteobacteria由4.4%分别提高到19.0%和12.8%.种植菹草后Verrucomicrobia所占比例大幅降低,而种植苦草对Verrucomicrobia的影响不大.沉水植物修复不仅可以改善水质,同时也可以显著提高水体中细菌的多样性,改变细菌的群落结构.

References

[1]  Le C,Zha Y,Li Y et al. Eutrophication of lake waters in China: cost,causes,and control. Environment Management, 2010,45(4): 662-668.
[2]  Smith VH,Schindler DW. Eutrophication science: where do we go from here? Ecology and Evolutionary Biology,2009, 24(4): 201-207.
[3]  Qin B. Lake eutrophication: Control countermeasures and recycling exploitation. Ecological Engineering,2009,35(11): 1569-1573.
[4]  种云霄,胡洪营,钱易. 大型水生植物在水污染治理中的应用研究进展. 环境污染治理技术与设备, 2003,4 (2): 36-40.
[5]  秦伯强. 长江中下游浅水湖泊富营养化发生机制与控制途径初探. 湖泊科学, 2002, 14(3): 193-202.
[6]  秦伯强. 长江中下游湖泊富营养化发生机制与控制对策. 中国科学院院刊,2 007,2 2(6): 503-505.
[7]  Cotner JB,Biddanda BA. Small players,large role: microbial influence on biogeochemical processes in pelagic aquatic ecosystems. Ecosystems,2002,5(2): 105-121.
[8]  Ciurli A,Zuccarini P,Alpi A. Growth and nutrient absorption of two submerged aquatic macrophytes in mesocosms,for reinsertion in a eutrophicated shallow lake. Wetlands Ecology and Management,2009,17(2): 107-115.
[9]  Rooney N,Kalff J. Submerged macrophyte-bed effects on water-column phosphorus,chlorophyll a,and bacterial production. Ecosystems,2003,6(8): 797-807.
[10]  吴根福,杨志坚,周雪平等. 杭州西湖水体中异养细菌生长的限制因子. 应用生态学报, 2003, 14(8): 1343-1346.
[11]  董百丽,秦伯强,龚志军等. 三种沉积物改良措施比较及其对苦草生长的影响. 生态学杂志,2011,30 (12): 2726-2731.
[12]  吴根福,吴洁. 杭州西湖水域微生物的生态调查. 水生生物学报,2 000,2 4(6): 589-596.
[13]  吴根福,虞左明,吴洁等. 杭州西湖水域可培养异养细菌的群落多样性. 生物多样性, 2003, 11(6): 467-474.
[14]  Amann RI,Ludwig W,Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews,1995,59(1): 143-169.
[15]  Muyzer G,De Waal EC,Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology, 1993,59(3): 695-700.
[16]  吴鑫,奚万艳,杨虹. 太湖梅梁湾冬季浮游细菌的多样性. 生态学杂志,2 006,2 5(10): 1196-1200.
[17]  奚万艳,吴鑫,叶文瑾等. 太湖梅梁湾水域蓝藻水华前与水华末期细菌群落结构的变化. 应用与环境生物学报, 2007, 13(1): 97-103.
[18]  Wu QL,Zwart G,Wu J et al. Submersed macrophytes play a key role in structuring bacterioplankton community composition in the large,shallow,subtropical Taihu Lake,China. Environmental Microbiology,2007,9(11): 2765-2774.
[19]  更多...
[20]  Tang X,Gao G,Chao J et al. Dynamics of organic-aggregate-associated bacterial communities and related environmental factors in Lake Taihu,a large eutrophic shallow lake in China. Limnology and Oceanography,2010,55(2): 469-480.
[21]  Vrede K. Nutrient and temperature limitation of bacterioplankton growth in temperate lakes. Microbial Ecology,2005,49 (2): 245-256.
[22]  Morris DP,Lewis WM. Nutrient limitation of bacterioplankton growth in Lake Dillon,Colorado. Limnology and Oceanography, 1992,37(6): 1179-1192.
[23]  Van Der Gucht K,Vandekerckhove T,Vloemans N et al. Characterization of bacterial communities in four freshwater lakes differing in nutrient load and food web structure. FEMS Microbiology Ecology,2005,53(2): 205-220.
[24]  冯胜,秦伯强,高光. 细菌群落结构对水体富营养化的响应. 环境科学学报,2 007, 27(11): 1823-1829.
[25]  Van Der Gucht K,Sabbe K,De Meester L et al. Contrasting bacterioplankton community composition and seasonal dynamics in two neighbouring hypertrophic freshwater lakes. Environmental Microbiology,2001,3(11): 680-690.
[26]  Dirnitriu PA,Pinkart HC,Peyton BM et al. Spatial and temporal patterns in the microbial diversity of a meromictic soda lake in Washington State. Applied and Environmental Microbiology,2008,74(15): 4877-4888.
[27]  孙鑫鑫,刘惠荣,冯福应等. 乌梁素海富营养化湖区浮游细菌多样性及系统发育分析. 生物多样性,2 009,1 7(5): 490-498.
[28]  李文红,施积炎. 西湖沉积物中解磷菌的分离纯化及其解磷能力. 应用生态学报, 2006, 17(11): 2112-2116.
[29]  邢鹏,孔繁翔,高光. 太湖浮游细菌种群基因多样性及其季节变化规律. 湖泊科学, 2007, 19(4): 373-381.
[30]  冯胜,高光,朱广伟等. 基于16S rDNA-DGGE 和FDC 技术对富营养化湖泊不同生态修复工程区细菌群落结构 的研究. 应用与环境生物学报, 2007,1 3(4): 535-540.
[31]  毛杰,邢鹏,刘正文. 惠州西湖沉水植被修复对浮游细菌群落结构的影响. 水生态学杂志, 2011, 32(3): 26-31.
[32]  金相灿,屠清瑛. 湖泊富营养化调查规范: 第2 版. 北京: 中国环境科学出版社,1 990.
[33]  高光,肖琳,杨柳燕等. 环境微生物实验技术. 北京: 中国环境科学出版社,2 004: 103-107.
[34]  Zhou J,Bruns MA,Tiedje JM. DNA recovery from soils of diverse composition. Applied and Environmental Microbiology, 1996,62(2): 316-322.
[35]  Schloss PD,Westcott SL,Ryabin T et al. Introducing mothur: open-source,platform-independent,community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology,2009,75(23): 7537-7541.
[36]  Kim OS,Cho YJ,Lee K et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology,2012,62: 716-721.
[37]  Crump BC,Hobbie JE. Synchrony and seasonality in bacterioplankton communities of two temperate rivers. Limnology and Oceanography,2005,50(6): 1718-1729.
[38]  Eiler A,Bertilsson S. Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environmental Microbiology,2004,6(12): 1228-1243.
[39]  Hahn MW. Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones. Applied and Environmental Microbiology,2003,69(9): 5248-5254.
[40]  Zwart G,Crump BC,Agterveld MPK-V et al. Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquatic Microbial Ecology,2002,28: 141-155.
[41]  Kasalicky V,Jezbera J,?imek K et al. Limnohabitans planktonicus sp. nov. and Limnohabitans parvus sp. nov.,planktonic betaproteobacteria isolated from a freshwater reservoir,and emended description of the genus Limnohabitans. International Journal of Systematic and Evolutionary Microbiology,2010,60(12): 2710-2714.
[42]  Hitchcock JN,Mitrovic SM,Kobayashi T et al. Responses of estuarine bacterioplankton,phytoplankton and zooplankton to dissolved organic carbon (DOC) and inorganic nutrient additions. Estuaries and Coasts,2010,33(1): 78-91.
[43]  Newton RJ,Jones SE,Eiler A et al. A guide to the natural history of freshwater lake bacteria. Microbiology and Molecular Biology Reviews,2011,75(1): 14-49.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133