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湖泊科学  2001 

促性腺激素释放激素和多巴胺对虹鳟(Oncorhynchusmykiss)离体脑垂体细胞分泌促性腺激素的影响

DOI: 10.18307/20010108

Keywords: 虹鳟,促性腺激素,促性腺激素释放激素,多巴胺,脑垂体细胞

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Abstract:

本文研究了不同性腺发育时期淡水饲养的雌雄虹鳟(Oncorhynchusmykiss)血浆促性腺激素(GTH)浓度的变化规律;以及在离体条件下,探讨了促性腺激素释放激素(GnRH)、GnRH拮抗物和多巴胺对虹鳟脑垂体细胞分泌促性腺激素(GTH-Ⅱ)的影响.结果表明:(1)在雄性,血浆中GTH-Ⅱ的水平随性腺发育而逐渐增高;在雌性,仅排卵期血浆中GTH-Ⅱ的水平明显增高.(2)未成熟期和成熟期的雌性虹鳟脑垂体细胞对sGnRH和cGnRH刺激的敏感性表现不尽相同.成熟期和排卵后的脑垂体细胞对GnRH的刺激较为敏感,GTH-Ⅱ的分泌量较多.(3)在没有sGnRH的存在下,GnRH拮抗物对GTH-Ⅱ的分泌不发生作用,而在sGnRH的存在下,GnRH拮抗物对GTH-Ⅱ的分泌表现出浓度依赖的抑制作用.(4)在没有sGnRH的存在下,多巴胺对GTH-Ⅱ的分泌不发生作用,而当多巴胺的浓度一定时,随着sGnRH浓度的增加,GTH-Ⅱ分泌的量增大.另外,不论sGnRH还是cGnRH都不影响不同时期的脑垂体细胞GTH-Ⅰ的分泌.这些结果可为虹鳟的人工催产提供一些理论依据.

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