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湖泊科学  2007 

太湖浮游细菌种群基因多样性及其季节变化规律

DOI: 10.18307/2007.0404

Keywords: PCR-DGGE,浮游细菌,基因多样性,季节变化,太湖

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Abstract:

为认识湖泊中浮游细菌的多样性及其变化规律,本研究采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)的方法,研究了太湖梅梁湾和湖心区浮游细菌种群16SrRNA基因多样性以及种群基因组成的季节变化特征。研究结果表明,太湖浮游细菌种群具有丰富的基因多样性。浮游细菌的丰度、DGGE条带数、种群的Shannon-Wiener指数、Simpson指数和Pielou指数均呈现显著的季节变化规律:夏季及秋季浮游细菌的丰度、基因多样性及种群的均匀度较高,而冬季和春季则较低。浮游细菌种群的基因组成随时间变化。梅梁湾浮游细菌的丰度显著高于湖心。梅梁湾与湖心浮游细菌主要建群种的基因型存在显著差异。

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