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湖泊科学  2007 

湖泊底泥中微囊藻DNA的分子检测

DOI: 10.18307/2007.0420

Keywords: 底泥,微囊藻,DNA,水华,蓝藻

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Abstract:

通过改进裂解温度和延长裂解时间并增加苯酚/氯仿洗脱次数的DNA提取方法获得南京玄武湖底泥中的DNA,通过PCR法来扩增微囊藻的16SrRNA基因。结果表明在所有采样点中均得到微囊藻基因组DNA,并且纯度较高,OD260/OD280均高于1.54,最高值达到1.89。PCR的扩增结果显示所有样点的DNA都得到212bp大小的微囊藻16SrRNA基因片断,表明这种方法可以有效的从底泥中提取微囊藻的DNA,从而为研究底泥微囊藻生理生态及其越冬、上浮、形成水华的机理提供更有利的方法。

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