全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
湖泊科学  2007 

鳙鱼微囊藻毒素去毒酶基因cDNA全序列的克隆与序列分析

DOI: 10.18307/2007.0315

Keywords: 微囊藻毒素去毒酶基因,cDNA序列,分子克隆,鳙鱼

Full-Text   Cite this paper   Add to My Lib

Abstract:

淡水鱼类可溶性谷胱甘肽S-转移酶(solubleglutathioneS-transferase,sGST)在微囊藻毒素去毒代谢过程中起着关键作用,又被称为微囊藻毒素去毒酶。通过简并引物克隆鳙鱼肝脏sGST基因cDNA核心序列,应用5’-RACE和3’-RACE技术分别扩增该序列的5’末端和3’末端序列,最后通过序列拼接获得鳙鱼肝脏sGST基因cDNA全序列。序列分析结果表明,鳙鱼肝脏sGST基因cDNA全长934bp,其中5’-UTR长104bp,3’-UTR长158bp,编码区672bp,编码223个氨基酸。鳙鱼与海水鱼类、鸟类、哺乳类等不同类群sGST的N-末端功能域氨基酸同源性较高,达75%左右,而C-末端功能域氨基酸同源性较低,仅为43.6%-55.9%,这与脊椎动物sGST的N-末端功能域均存在相同的GSH结合位点,而C-末端功能域存在不同的底物结合位点相一致。鳙鱼sGST的C-末端功能域的低同源性可能与其承担微囊藻毒素去毒代谢之特殊功能有关。

References

[1]  Carmichael W W.Health effects of toxin-producing cyanobacteria:the CyanoHABs\' Hum Ecol Risk Assess,2001,7:1393-1407.
[2]  Yu S J.Primary prevention of hepatocelluar carcinoma.J Gastroenterol Hepatol,1995,10:674-682.
[3]  Pouria S,Andrade A D,Barbosa J et al.Fatal microcystin intoxication in haemodialysis unit in Caruaru,Brazil.Lancet,1998,352:21-26.
[4]  Hitzfeld B C,Hoger S J,Dietrich R.Cyanobacterial toxins:removal during drinking water treatment,and human risk assessment.Environ Health Perspect,2000,10:113-122.
[5]  谢平.水生动物体内的微囊藻毒素及其对人类健康的潜在威胁.北京:科学出版社,2006.
[6]  Dirr H,Reinemer P,Huber R.X-ray crystal structures of cytosolic glutathione S-transferases Implications for protein architecture,substrate recognition and catalytic function.Eur J Biochem,1994,220:645-661.
[7]  Whalen R,Boyer T.Human glutathione S-transferase.Semin Liver Dis,1998,18(4):345-358.
[8]  谢平.鲢、鳙与藻类水华控制.北京:科学出版社,2003.
[9]  聂立红,王声勇,胡毅玲.谷胱甘肽硫-转移酶研究进展.中国病理生理杂志,2000,16(11):1240-1243.
[10]  Oakley A J,Lo Bello M,Nuccetelli M et ah The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile bindingsite (H site).J Mol Biol,1999,291(4):913-926.
[11]  Codd G A.Cynobacterial toxins:occurrence,properties and biological significance.Water Sci Technol,1995,32:149-156.
[12]  Dawson R M.The toxicology of microcystins.Toxicon,1998,36:953-962.
[13]  Chorus I,Falconer I R,Salas H J et al.Health risks caused by freshwater cyanobacteria in recreational water.J Toxicol Environ Health,2000,3:323-347.
[14]  Hayes J D,Flanagan J U,Jowsey I R.Glutathione transferases.Annu Rev Pharmacol Toxicol,2004 ,45:51-88.
[15]  Li J,Xia Z X,Ding J P.Thioredoxin-like domain of human K class glutathione transferase reveals sequence homology and structure similarity to the y class enzyme.Protein Sci,2005,14:2361-236.
[16]  Sheehan D,Casey J P.Microbial glutathione S-transferases.Com Biochem Physiol,1993,104:1-6.
[17]  Best J H,Eddy F B,Codd G A.Effects of Microcystis cells,cell extracts and lipopolysaccharide on drinking and liver function in rainbow trout Oncorhynchus mykiss Walbaum.Aquat Toxicol,2003,64:419-426.
[18]  Wiegand C,Pfugmacher S,Giese M et al.Uptake,toxicity,and effects on detoxication enzymes of atrazine and trifluoroacetate in embryos of zebrafish.Ecotoxicol Environ Saf,2000,45:122-131.
[19]  Adriana M Doil,Robert T Pham,Erin M Hughes,David S Barber,Evan P.Gallagher Molecular cloning and characterization of a glutathione S-transferase from largemouth bass (Microptems salmoides) liver that is involved in the detoxification of 4-hydroxynonenal.Biochemical Pharmacology,2004,67:2129-2139.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133