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湖泊科学  2012 

安徽沿江两个富营养化浅水湖泊克氏原螯虾(Procambarusclarkii)遗传毒性比较

DOI: 10.18307/2012.0118

Keywords: 遗传毒性,微核实验,彗星实验,巢湖,富营养化湖泊,克氏原螯虾,菜子湖

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Abstract:

富营养化湖泊的蓝藻水华加剧了水体污染,影响湖泊生态系统的稳定.本研究针对安徽沿江富营养化程度不同的巢湖和菜子湖,2010年49月份,利用微核和彗星电泳实验检测水体污染物对克氏原螯虾(Procambarusclarkii)血细胞的微核率和DNA损伤,评价湖泊污染物的遗传毒性效应.巢湖和菜子湖细胞微核率最低值分别为0.316±0.131和0.243±0.063,最高值分别为0.624±0.136和0.360±0.081;巢湖DNA损伤三项指标TailDNA、TM和OTM最高值为7.59±1.58、2.75±1.55和3.12±1.45,菜子湖为5.02±1.42、1.07±0.16和1.19±0.26.两个湖泊克氏原螯虾血细胞微核率和DNA损伤都表现为随时间推移而增加,且巢湖6、7月份血细胞微核率和DNA损伤增加幅度最大.7、8、9三个月份,两湖泊细胞微核率和DNA损伤差异性达到极显著.彗星实验和微核实验结果一致,但前者更敏感.两种不同的遗传毒性检测方法结合能更好地检测污染物对机体产生的遗传毒性作用.

References

[1]  Lacaze E,Geffard O,Bony S et al. Genotoxicity assessment in the amphipod Gammarus fossarum by use of the alkaline Comet assay. Mutation Research,2010,700:32-38.
[2]  Wang YM,Chen P,Cui RN et al. Heavy metal concentrations in water,sediment,and tissues of two fish species (Triplohysa pappenheimi,Gobio hwanghensis) from the Lanzhou section of the Yellow River,China. Environmental Monitoring Assessment,2010,165:97-102.
[3]  Faria M,López MA,Fernández-Sanjuan M. Comparative toxicity of single and combined mixtures of selected pollutants among larval stages of the native freshwater mussels (Unio elongatulus) and the invasive zebra mussel (Dreissena polymorpha). Science of the Total Environment,2010,408:2452-2458.
[4]  Zhang M,Xu J,Xie P et al. Metals in surface sediments of large shallow eutrophic Lake Chaohu. Bulletin of Environmental Contamination and Toxicology,2007,79:242-245.
[5]  Terry SF. Genetic testing and biomarkers in the new decade. Genetic Testing and Molecular Biomarkers,2010,14(1):1-2.
[6]  Klobucar GIV,Pavlica M,Erben R et al. Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquatic Toxicology,2003,64:15-23.
[7]  Yin XH,Li SN,Zhang L et al. Evaluation of DNA damage in Chinese toad (Bufo bufo gargarizans) after in vivo exposure to sublethal concentrations of four herbicides using the comet assay. Ecotoxicology,2008,17:280-286.
[8]  Jin LN,Zhang WH,Zheng L et al. Biodegradation of microcystin in Dianchi Lake aquatic environment. China Environmental Science,2002,22(2):189-192.
[9]  Zhang DW,Xie P,Liu YQ et al. Transfer,distribution and bioaccumulation of microcystins in the aquatic food web in Lake Taihu,China,with potential risks to human health. Science of the Total Environment,2009,407:2191-2199.
[10]  Dorr FA,Pinto E,Soares RM et al. Microcystins in South American aquatic ecosystems:Occurrence,toxicity and toxicological assays. Toxicon,2010,56:1247-1256.
[11]  Chen X,Yang XD,Dong XH et al. Nutrient dynamics linked to hydrological condition and anthropogenic nutrient loading in Lake Chaohu (southeast China). Hydrobiologia,2011,661:223-234.
[12]  Froehner S,Maceno M. Assessment of bioaccumulation of biphenyls in the trophic chain of a coastalareaof Parana,Brazil. Environmental Monitoring Assessment,2010,164:189-198.
[13]  Webster L,Russell M,Walsham P et al. An assessment of persistent organic pollutants (POPs) in wild and rope grown blue mussels (Mytilius edulis) from Scottish coastal waters. Journal of Environmental Monitoring,2009,11:1169-1184.
[14]  赵顺顺,孟范平,王震宇等. 监测水体重金属污染的分子生物标志物研究进展. 生态环境学报,2010,19(2):453-458.
[15]  Faria M,López MA,Fernández-Sanjuan M et al. Comparative toxicity of single and combined mixtures of selected pollutants among larval stages of the native freshwater mussels (Unio elongatulus) and the invasive zebra mussel (Dreissena polymorpha). Science of the Total Environment,2010,408:2452-2458.
[16]  冯承莲,雷炳莉,王子健. 中国主要河流中多环芳烃生态风险的初步评价. 中国环境科学,2009,29(6):583-588.
[17]  张明,花日茂,李学德等. 巢湖表层水体中有机氯农药的分布及其组成. 应用生态学报,2010,21(1):209-214.
[18]  Jha AN. Genotoxicological studies in aquatic organisms:an overview. Mutation Research,2004,552:1-17.
[19]  Andrade VM,de Freitas TRO,da Silva J. Comet assay using mullet (Mugil sp.) and sea catfish (Netuma sp.) erythrocytes for the detection of genotoxic pollutantsin aquatic environment. Mutation Research,2004,560:57-68.
[20]  Frenzilli G,Nigro M,Lyons BP. The comet assay for the evaluation of genotoxic impact in aquatic environments. Mutation Research,2009,681:80-92.
[21]  Mai WJ,Yan JL. Acute acidic exposure induces p53-mediated oxidative stress and DNA damage in tilapia (Oreochromis niloticus) blood cells. Aquatic Toxicology,2010,100:271-281.
[22]  Boettcher M,Grund S,Keiter S et al. Comparison of in vitro and in situ genotoxicity in the Danube River by means ofthe comet assay and the micronucleus test. Mutation Research,2010,700:11-17.
[23]  Zhang DW,Xie P,Liu YQ et al. Spatial and temporal variations of microcystins in hepatopancreas of a freshwater snail from Lake Taihu. Ecotoxicology and Environmental Safety,2009,72:466-473.
[24]  周立志,陈春玲. 三种重金属在克氏原螯虾体内的富集特征. 生态学杂志,2008,27(9):1498-1502.
[25]  Jin XC,Xu QJ,Huang CZ. Current status and future tendency of lake eutrophication in China. Life Science,2005,48:948-954.
[26]  Dhawan A,Bajpayee M,Parmar D. Comet assay:a reliable tool for the assessment of DNA damage in different models. Cell Biology Toxicology,2009,25:5-32.
[27]  更多...
[28]  李兆利,厉以强,陈海刚等. 长江南京段水体中有机污染物的遗传毒性研究. 环境科学研究,2006,19(3):105-108.
[29]  Rosa R,Rodea-Palomares I,Boltes K et al. Ecotoxicological assessment of surfactants in the aquatic environment:Combined toxicity of docusate sodium with chlorinated pollutants. Chemosphere,2010,81:288-293.
[30]  Xu MQ,Cao H,Xie P et al. The temporal and spatial distribution,composition and abundance of Protozoa in Lake Chaohu,China:Relationship with eutrophication. European Journal of Protistology,2005,41:183-192.
[31]  黄德军. 黄河兰州段水质污染对花背蟾蜍的毒理学影响[学位论文]. 兰州:兰州大学,2007.
[32]  Li M,Hu CW,Gao XY et al. Genotoxicity of organic pollutants in source of drinking water on microalga Euglena gracilis. Ecotoxicology,2009,18:669-676.
[33]  Xie XY,Qian X,Zhang YC et al. Effect on Lake Chaohu water environment of water transfer from Yangtze River to Lake Chaohu. Research of Environmental Sciences,2009,22(8):897-901.
[34]  Correia AM. Food choice by the introduced crayfish Procambarus clarkii. Annales Zoologic Fennici,2003,40:517-528.
[35]  童军华,黄祥明,陈勇. 巢湖水体重金属污染评价. 安徽农业科学,2006,34(17):4373-4374.
[36]  宋维彦,靳桂双,毕伟伟等. 五种重金属离子对克氏原螯虾(Procambarus clarkii) 的急性毒性作用研究. 激光生物学报,2010,19(2):201-211.
[37]  Engraff M,Solere C,Smith KE. Aquatic toxicity of PAHs and PAH mixtures at saturation to benthic amphipods:Linking toxic effects to chemical activity. Aquatic Toxicology,2011,102:142-149.
[38]  邹爱红. 巢湖西半湖水体中有机污染物监测及污染现状的研究[学位论文]. 合肥:合肥工业大学,2009.
[39]  王永华,徐福留. 顶空气相色谱法测定水中挥发性有机污染物. 湖泊科学,2010,22(6):910-915.
[40]  Kim SG,Joung SH,Ahn CY et al. Annual variation of Microcystis genotypes and their potential toxicity in water and sediment from a eutrophic reservoir. Microbiology Ecology,2010,4:93-102.
[41]  杨华. 巢湖和太湖微囊藻毒素的生态学研究[学位论文]. 武汉:中国科学院水生生物研究所,2006.
[42]  Juhel G,O\'Halloran J,Culloty SC et al. In vivo exposure to microcystins induces DNA damage in the haemocytes of the Zebra mussel,Dreissena polymorpha,as measured with the comet assay. Environmental and Molecular Mutagenesis, 2007,48:22-29.
[43]  Zhong Y,Feng SL,Luo Y et al. Evaluating the genotoxicity of surface water of Yangzhong city using the Vicia Faba micronucleus test and the comet assay. Bulletin of Environment Contammintion Toxicology,2001,67:217-224.
[44]  Ali FK,El-Shehawi AM,Seehy MA. Micronucleus test in fish genome:A sensitive monitor for aquatic pollution. African Journal of Biotechnology,2008,7(5):606-612.

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