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湖泊科学  2002 

Zn2+浓度对固氮鱼腥藻(AnabaenaazoticaLey)光能转化特性的影响

DOI: 10.18307/2002.0409

Keywords: 固氮鱼腥藻,锌离子,光合作用,藻蓝蛋白,碳酸酐酶

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Abstract:

本实验对在不同Zn2+浓度条件下培养的固氮鱼腥藻(AnabaenaazoticaLey)的生长、光合放氧速率和叶绿素荧光参数Fv/Fm进行了测定.结果表明,当Zn2+浓度为1.0μmol/L时,其比生长速率(Specificgrowthrate)最大,光合放氧速率和Fv/Fm值最高.当Zn2+浓度大于等于5.0μmol/L时会抑制A.azoticaLey的生长和光合作用.对在0μmol/L和5.0μmol/LZn2+浓度下生长的藻细胞藻胆体-类囊体膜复合物吸收光谱的比较和对与5.0μmol/LZn2+发生反应的藻蓝蛋白溶液的可见光吸收光谱的分析,发现前者624nm处藻胆体的吸收峰和后者620nm处藻蓝蛋白的吸收峰都因Zn2+的作用而下降,推测藻蓝蛋白为Zn2+影响光合作用的位点之一.碳酸酐酶活性的测定表明Zn2+的浓度水平会影响其活性大小,推测是Zn2+影响光合作用的另-途径.

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