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湖泊科学  2008 

微囊藻衰亡过程研究——四种模拟胁迫条件下微囊藻的衰亡生理

DOI: 10.18307/2008.0501

Keywords: 微囊藻,衰亡,磷饥饿,胁迫,抗氧化酶系统

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Abstract:

研究了四种胁迫条件下微囊藻衰亡过程以及衰亡过程中生长生理与抗氧化酶系统的变化以及胁迫30d后转入正常培养后的微囊藻的生长变化过程.将对数生长期的微囊藻分别转入氮饥饿、磷饥饿、黑暗及10℃低温等胁迫条件下培养,测定光密度OD680、叶绿素a、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性等生理指标.结果表明,在黑暗和低温条件下,微囊藻生长受到明显抑制,但黑暗条件下25d后微囊藻衰亡,低温条件下仅需7d;与物理因子引起的胁迫相比,在营养胁迫条件下,微囊藻在胁迫前期均表现出生长趋势.N饥饿条件下叶绿素a含量从开始胁迫后即下降,P饥饿20d后叶绿素a含量以0.1mg/(L·d)急剧下降,在四种胁迫条件中下降速率最高,表明微囊藻对P的耐受能力最差.根据实验结果,在长期没有外来P源供给而内部又无可得P源的水体中、或者水温急剧下降到10℃以下时,微囊藻水华可能会快速衰亡,相应的应急措施应准备到位,以尽量减轻水体微囊藻水华衰亡带来的损失.SOD与CAT活性的上升与叶绿素a含量的下降存在显著相关性,可以尝试将两者结合预测微囊藻水华的衰亡.本研究在实验室条件下初步探讨了四种环境因子对微囊藻消亡的贡献,比较了SOD与CAT活性与叶绿素a含量下降的相关性,为微囊藻水华衰亡预测体系的建立提供了理论依据.

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