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湖泊科学  2009 

太湖梅梁湾水华微囊藻基因型组成和产毒微囊藻丰度的变化

DOI: 10.18307/2009.0608

Keywords: 微囊藻,产毒微囊藻,ITS,实时荧光定量PCR,DGGE,水华,太湖

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Abstract:

采用基于ITS序列的PCR-DGGE方法分析了太湖梅梁湾水华暴发过程中(6-11月)微囊藻的不同基因型组成的变化,同时利用针对微囊藻毒素合成酶基因mcyA和微囊藻特异性的16SrDNA部分核苷酸序列的引物,应用实时荧光定量PCR方法(qRT-PCR)分析水华样品中产毒微囊藻与总微囊藻的量,通过两者的比值反映产毒微囊藻丰度.结果表明,在发生水华的不同时期,微囊藻的基因型组成发生了变化,其中以8月末、9月和10月的基因型最多,基因型M5和M10存在于整个水华发展过程中.共检测到12种主要的基因型,每个基因型在水华的不同时期所占的比例互不相同.实时荧光定量PCR结果显示从6月到10月,产毒微囊藻的丰度呈增大的趋势,从0.75%增加到32.16%,而11月产毒微囊藻的丰度显著下降.

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