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未培养微生物研究的两种新技术及其寻找功能基因的应用

DOI: 10.11830/ISSN.1000-5013.2008.02.0177

Keywords: 未培养微生物, 宏基因组, 稳定同位素, 功能基因, 微生物生态

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Abstract:

在微生物筛选过程中,99%的未培养微生物是用传统的方法无法分离的,这极大地限制了微生物生态领域中的多样性和利用生物资源等方面的研究.采用新技术成为研究未培养微生物的技术瓶颈.综述应用于未培养微生物的两种新技术――宏基因组和稳定同位素,探讨这两种新技术在寻找功能基因中的应用,特别是在生态环境中寻找功能微生物.探讨降解微生物及其功能基因对污染的生态环境进行的生物修复,为进一步研究微生物生态多样性及其功能基因的开发应用打下基础.

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