全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...

生物酶降解米糠释放阿魏酸工艺条件优化

DOI: 10.11830/ISSN.1000-5013.2011.01.0062

Keywords: 阿魏酸, 米糠, 均匀设计, 木聚糖酶, 生物降解

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用均匀设计法设计和二次多项式逐步回归分析,优化一株放线菌降解米糠释放反式阿魏酸的培养基配方,并采用单因素法优化发酵条件.优化后培养基配方:米糠质量分数为6%,米糠粒径0.50mm,酵母粉质量分数为1.0%; 优化后的发酵条件:发酵温度为28℃,摇床转速200r.min-1,装液系数为12%,接种量的体积分数为3.3%,pH值8.0.实验结果表明,反式阿魏酸的释放率最高为14.55%,与优化前相比,释放率提高了106.7%.研究阿魏酸酯酶和木聚糖酶联合作用于米糠产阿魏酸和低聚糖的协同效应,阿魏酸释放率达到20.22%,与阿魏酸酯酶单独降解米糠相比,其释放率提高了119.1%; 低聚木糖的释放量2.04mg.mL-1,与木聚糖酶单独降解米糠相比,其释放量提高了87.2%.

 References

[1]  YU P Q, MCKINNON J J, CHRISTENSEN D A. Hydroxycinnamic acids and ferulic acid esterase in relation to biodegradation of complex plant cell walls [J]. Canadian Journal of Animal Science, 2005, (3):255-267.
[2]  YU P Q, MCKINNON J J, CHRISTENSEN D A. Hydroxycinnamic acids and ferulic acid esterase in relation to biodegradation of complex plant cell walls [J]. Canadian Journal of Animal Science, 2005(3):255-267.
[3]  SISDHU MATHEW, T EMILIA ABRAHAM. Ferulic acid:An antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications [J]. Critical Reviews in Biotechnology, 2004, (2/3):59-83.
[4]  SISDHU MATHEW, T EMILIA ABRAHAM. Ferulic acid:An antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications [J]. Critical Reviews in Biotechnology, 2004, (2/3):59-83.doi:10.1080/07388550490491467.
[5]  YU P Q, MAENZ D D, MCKINNON J J. Release of ferulic acid from oat hulls by Aspergillus ferulic acid esterase and Trichoderma xylanase [J]. Journal of Agricultural and Food Chemistry, 2002, (6):1625-1630.
[6]  YU P Q, MAENZ D D, MCKINNON J J. Release of ferulic acid from oat hulls by Aspergillus ferulic acid esterase and Trichoderma xylanase [J]. Journal of Agricultural and Food Chemistry, 2002(6):1625-1630.
[7]  BARTOLOME B, FAULDS C B, WILLIAMSON G. Enzymic release of ferulic acid from barley spent grain [J]. Journal of Cereal Science, 1997, (3):285-288.
[8]  BARTOLOME B, FAULDS C B, WILLIAMSON G. Enzymic release of ferulic acid from barley spent grain [J]. Journal of Cereal Science, 1997(3):285-288.doi:10.1006/jcrs.1996.0091.
[9]  张？, 欧仕益. 酶解麦麸制备阿魏酸和低聚糖的研究 [J]. 食品科学, 2003, (11):63-68.doi:10.3321/j.issn:1002-6630.2003.11.012.
[10]  张？, 欧仕益, 张宁, 郑洁. 酶解麦麸制备阿魏酸和低聚糖的研究 [B]. 食品科学, 2003, (11):63-68.
[11]  黄艳凤, 周庆礼, 李士炼. 从黑麦麦麸中提取的阿魏酸的检测 [J]. 药物生物技术, 2004(1):49-51.doi:10.3969/j.issn.1005-8915.2004.01.013.
[12]  黄艳凤, 周庆礼, 李士炼, 戴丙业. 从黑麦麦麸中提取的阿魏酸的检测 [A]. 药物生物技术, 2004, (1):49-51.
[13]  张国升. 从米糠中提取阿魏酸的研究 [J]. 安徽中医学院学报, 1997(6):56-57.
[14]  张国升. 从米糠中提取阿魏酸的研究 [J]. 安徽中医学院学报, 1997, (6):56-57.
[15]  唐启义, 冯明光. 实用统计分析及其DPS数据处理系统 [M]. 北京:科学出版社, 2002.
[16]  唐启义, 冯明光. 实用统计分析及其DPS数据处理系统 [M]. 北京:科学出版社, 2002.
[17]  BERZILLON C, KROON P A, FAULDS C B. Novel ferulic acid esterases are induced by growth of Aspergillus niger on sugar beet pulp [J]. Appl Biotechnol, 1996, (3):371-376.
[18]  BERZILLON C, KROON P A, FAULDS C B. Novel ferulic acid esterases are induced by growth of Aspergillus niger on sugar beet pulp [J]. Appl Biotechnol, 1996(3):371-376.
[19]  TOPAKAS E, VAFIADI C, STAMATIS H. Sporotrichum thermophile type C feruloyl esterase (StFaeC):Purification, characterization, and its use for phenolic acid (sugar) ester synthesis [J]. Enzyme and Microbial Technology, 2005, (5/6):729-736.doi:10.1016/j.enzmictec.2004.12.020.
[20]  TOPAKAS E, VAFIADI C, STAMATIS H. Sporotrichum thermophile type C feruloyl esterase (StFaeC):Purification, characterization, and its use for phenolic acid (sugar) ester synthesis [J]. Enzyme and Microbial Technology, 2005, (5/6):729-736.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133