全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

??????澧????Cry7Ab4??????????????

DOI: 10.11830/ISSN.1000-5013.2012.01.0060

Keywords: ??????????, ??澧????, Cry7Ab4, ????, ??Ч???

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用一级结构分析,二级结构预测及同源比对建模的方法,模拟出新型杀虫晶体蛋白Cry7Ab4的空间结构,并进行动力学优化.结合其杀大猿叶甲活性数据与蛋白结构比对分析,发现Cry7Ab4与Cry7Aa1的活性差异主要归因于α3,α4上疏水性氨基酸的不同.

References

[1]  SONG L, GAO M, DAI S. Specific activity of a Bacillus thuringiensis strain against Locusta migratoria manilensis [J]. Journal of Invertebrate Pathology, 2008(2):169-176.doi:10.1016/j.jip.2008.02.006.
[2]  邓淑, 束长龙, 林毅. 新型Cry7Ab基因的鉴定、克隆、表达与杀虫活性J] [J]. 农业生物技术学报, 2009.908-913.
[3]  SCHNEPF E, CRICKMORE N, VAN RIE J L. Bacillus thuringiensis and its pesticidal crystal protein [J]. Microbiol and Molecular Biology Review, 1998(3):775-806.
[4]  LI J, CARROLL J, ELLAR D J. Crystal structure of insecticide δ-endotoxin from Bacillus thuringiensis at 2.5 (A)resolution [J]. Nature, 1991.815-821.doi:10.1038/353815a0.
[5]  CHOE S, BENNETT M J, FUJII G. The crystal structure of diphtheria toxin [J]. Nature, 1992.216-222.doi:10.1038/357216a0.
[6]  OJCIUS D M, YOUNG J D K. Cytolytic pore-forming proteins and peptides:Is there a common structural motif [J]. Trends in Biochemical Sciences, 1991(6):225-229.
[7]  YOOL A J. Block of the inactivating potassium channel by clofilium and hydroxylamine depends on the sequence of the pore region [J]. Molecular Pharmacology, 1994(5):970-976.
[8]  LAMBERT B, THEUNIS W, AGUDA R. Nucleotide sequence of gene cry Ⅲ D encoding a novel coleopteranactive crystal protein from strain BTI109P of Bacillus thuringiensis subsp.kurstaki [J]. Gene, 1992(1):131-132.doi:10.1016/0378-1119(92)90457-Z.
[9]  SCHWARTZ J L, JUTEAU M, GROCHULSKI P. Restriction of intramolecular movements within the CrylAa toxin molecule of Bacillus thuringiensis through disulfide bond engineering [J]. FEBS Letters, 1997, (2/3):397-402.doi:10.1016/S0014-5793(97)00626-1.
[10]  YAOI K, NAKANISHI K, KADOTANI T. Bacillus thuringiensis CrylAa toxin-binding region of Bombyx mori aminopeptidase N [J]. FEBS Letters, 1999(3):221-224.doi:10.1016/S0014-5793(99)01626-9.
[11]  MASSON L, MAZZA A, GRINGORTEN L. Specificity domain localization of Bacillus thuringiensis insecticidal toxins is highly dependent on the bioassay system [J]. Molecular Microbiology, 1994(5):851-860.doi:10.1111/j.1365-2958.1994.tb01321.x.
[12]  GERBER D, SHAI Y. Insertion and organization within membranes of the δ-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide [J]. Journal of Biological Chemistry, 2000, (31):23602-23607.
[13]  HODGMAN T C, ELLAR D J. Models for the structure and function of the Bacillus thuringiensis δ-endotoxins determined by compilational analysis [J]. Dna Sequence, 1990(2):97-106.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133