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基于DNA双链增强量子点自组装膜能量转移的界面比率传感

DOI: 10.11830/ISSN.1000-5013.2013.05.0529

Keywords: 能量转移, 自组装膜, 界面荧光, 比率传感, 脱氧核糖核酸

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Abstract:

分别以巯基乙酸(TGA)和三聚磷酸钠(STPP)为稳定剂,在水相条件下制备CdTe量子点和ZnO@CdS量子点.基于DNA双链增强自组装膜能量转移的原理,以ZnO@CdS量子点为能量供体,CdTe量子点为能量受体,构筑Quartz/PDDA/CdTe/PDDA/T1-DNA/PDDA/ZnO@CdS SAMs自组装膜,并实现对DNA的界面比率传感.随着匹配DNA(P1-DNA)的加入,ZnO@CdS量子点的荧光强度FD减小,相对荧光强度FA/FD和P1-DNA浓度的负对数呈线性关系,线性范围为8.686×10-9~6.080×10-8 mol?L-1,检测限为0.707 nmol?L-1.

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