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RNAi表达载体在哺乳动物中的研究进展

DOI: 10.11830/ISSN.1000-5013.2015.03.0314, PP. 314-320

Keywords: RNA干扰,表达载体,短发夹式RNA载体,微小RNA载体,哺乳动物

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Abstract:

对RNA干扰(RNAi)表达载体的非生物与生物递送系统及主流应用进行综述,明确其在基因调控及未来基因治疗领域的应用前景,并指出其在种属特异性及表达效率等方面存在的局限性.对RNAi表达载体的结构组成、发展沿革及体内作用机制进行归纳分析,提出基于结构组成和加工机制双重属性的分类方法.

References

[1]  MACDIARMID J A,AMARO-MUGRIDGE N B,MADRID-WEISS J,et al.Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug[J].Nature Biotechnology,2009,27(7):643-651.
[2]  ANDERSSON M G,HAASNOOT P J,XU N,et al.Suppression of RNA interference by adenovirus virus-associated RNA[J].Journal of Virology,2005,79(15):9556-9565.
[3]  卢善良,黄轶群,马旭东.下调组蛋白去乙酰化酶1的表达引起人白血病HL-60细胞的分化[J].药学学报,2013,48(5):668-674.
[4]  BOREL F,van LOGTENSTEIN R,KOORNNEEF A,et al.In vivo knock-down of multidrug resistance transporters ABCC1 and ABCC2 by AAV-delivered shRNAs and by artificial miRNAs[J].J RNAi Gene Silencing,2011,7:434-442.
[5]  van ES H H,ARTS G J.Biology calls the targets: Combining RNAi and disease biology[J].Drug Discovery Today,2005,10(20):1385-1391.
[6]  HUANG H Y,CHIANG B L.siRNA as a therapy for asthma[J].Curr Opin Mol Ther,2009,11(6):652-663.
[7]  LI Yang,LU Jin-feng,HAN Yan-hong,et al.RNA interference functions as an antiviral immunity mechanism in mammals[J].Science,2013,342(6155):231-234.
[8]  BRUMMELKAMP T R,BERNARDS R,AGAMI R A.System for stable expression of short interfering RNAs in mammalian cells[J].Science,2002,296(5567):550-553.
[9]  ZENG Y,WAGNER E J,CULLEN B R.Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells[J].Molecular Cell,2002,9(6):1327-1333.
[10]  van GESTEL M A,van ERP S,SANDERS L E,et al.shRNA-induced saturation of the microRNA pathway in the rat brain[J].Gene Therapy,2014,21(2):205-211.
[11]  FELLMANN C,ZUBER J,MCJUNKIN K,et al.Functional identification of optimized RNAi triggers using a massively parallel sensor assay[J].Molecular Cell,2011,41(6):733-746.
[12]  PADDISON P J,SILVA J M,CONKLIN D S,et al.A resource for large-scale RNA-interference-based screens in mammals[J].Nature,2004,428(6981):427-431.
[13]  BOUDREAU R L,MONTEYS A M,DAVIDSON B L.Minimizing variables among hairpin-based RNAi vectors reveals the potency of shRNAs[J].Rna,2008,14(9):1834-1844.
[14]  STEGMEIER F,HU G,RICKLES R J,et al.A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(37):13212-13217.
[15]  ZHOU J,LI B,PENG C,et al.Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes[J].Antiviral Research,2013,98(2):305-313.
[16]  CHUMAKOV S P,KRAVCHENKO J E,PRASSOLOV V S,et al.Efficient downregulation of multiple mRNA targets with a single shRNA-expressing lentiviral vector[J].Plasmid,2010,63(3):143-149.
[17]  VIDIGAL J A,MORKEL M,WITTLER L,et al.An inducible RNA interference system for the functional dissection of mouse embryogenesis[J].Nucleic Acids Research,2010,38(11):e122.
[18]  DOW L E,PREMSRIRUT P K,ZUBER J,et al.A pipeline for the generation of shRNA transgenic mice[J].Nature Protocols,2012,7(2):374-393.
[19]  WHEELER L A,VRBAMAC V,TRIFONOVA R,et al.Durable knockdown and protection from HIV transmission in humanized mice treated with gel-formulated CD4 aptamer-siRNA chimeras[J].Molecular Therapy: The Journal of the American Society of Gene Therapy,2013,21(7):1378-1389.
[20]  KOZOMARA A,GRIFFITHS J S.Mirbase: Integrating microRNA annotation and deep-sequencing data[J].Nucleic Acids Research,2011(39):D152-D157.
[21]  SCHOPMAN N C,LIU Y P,KONSTANTINOVA P,et al.Optimization of shRNA inhibitors by variation of the terminal loop sequence[J].Antiviral Research,2010,86(2):204-211.
[22]  CHUMAKOV S P,KRAVCHENKO J E,PRASSOLOV V S,et al.Efficient downregulation of multiple mRNA targets with a single shRNA-expressing lentiviral vector[J].Plasmid,2010,63(3):143-149.
[23]  WU Jun-zhu,LIU Shi-he,YU Jian,et al.Vertically integrated translational studies of PDX1 as a therapeutic target for pancreatic cancer via a novel bifunctional RNAi platform[J].Cancer Gene Ther,2014,21(2):48-53.
[24]  WU Jun-zhu,BONSRA A N,DU Guang-wei.pSM155 and pSM30 vectors for miRNA and shRNA expression[M]. Totowa:Humana Press,2009:1-15.
[25]  FELLMANN C,HOFFMANN T,SRIDHAR V,et al.An optimized microRNA backbone for effective single-copy RNAi[J].Cell Reports,2013,5(6):1704-1713.
[26]  YUE Jun-ming,SHENG Yi,REN Ai-xia,et al.A miR-21 hairpin structure-based gene knockdown vector[J].Biochemical and Biophysical Research Communications,2010,394(3):667-672.
[27]  CHEN S C Y,STERN P,GUO Zhu-yan,et al.Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector[J].PloS One,2011,6(7):e22437.
[28]  LEE N S,DOHJIMA T,BAUER G,et al.Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells[J].Nature Biotechnology,2002,20(5):500-505.
[29]  CHEN Chang-zhen,LI Ling,LODISH H F,et al.MicroRNAs modulate hematopoietic lineage differentiation[J].Science,2004,303(5654):83-86.
[30]  SILVA J M,LI M Z,CHANG K,et al.Second-generation shRNA libraries covering the mouse and human genomes[J].Nat Genet,2005,37(11):1281-1288.
[31]  MOFFAT J,GRUENEBERG D A,YANG X,et al.A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen[J].Cell,2006,124(6):1283-1298.
[32]  BOUDREAU R L,MARTINS I,DAVIDSON B L.Artificial microRNAs as siRNA shuttles: Improved safety as compared to shRNAs in vitro and in vivo[J].Molecular Therapy:The Journal of the American Society of Gene Therapy,2009,17(1):169-175.
[33]  CHUNG K H,HART C C,AL-BASSAM S,et al.Polycistronic RNA polymerase Ⅱ expression vectors for RNA interference based on BIC/miR-155[J].Nucleic Acids Research,2006,34(7):e53.
[34]  WILSON R C,DOUDNA J A.Molecular mechanisms of RNA interference[J].Annual Review of Biophysics,2013,42:217-239.
[35]  陈光伟,王士斌.纳米载体共载基因与化疗药物用于癌症治疗的研究进展[J].药学学报,2013,48(7):1091-1098.
[36]  QAZI Y,STAGG B,SINGH N,et al.Nanoparticle-mediated delivery of shRNA.VEGF-a plasmids regresses corneal neovascularization[J].Investigative Ophthalmology and Visual Science,2012,53(6):2837-2844.
[37]  HAN Lu,TANG Cui,YIN Chun-hua.Oral delivery of shRNA and siRNA via multifunctional polymeric nanoparticles for synergistic cancer therapy[J].Biomaterials,2014,35(15):4589-4600.
[38]  陆艳梅,张金文,魏玉洁,等.罂粟COR和BBE基因RNAi载体构建及烟草转化[J].药学学报,2013,48(7):1169-1177.
[39]  MACDIARMID J A,MUGRIDGE N B,WEISS J C,et al.Bacterially derived 400 nm particles for encapsulation and cancer cell targeting of chemotherapeutics[J].Cancer Cell,2007,11(5):431-445.
[40]  KEATES A C,FRUEHAUF J H,XIANG Shuang-lin,et al.Cequent Pharmaceuticals, Inc: The biological pitcher for RNAi therapeutics[J].Pharmacogenomics,2007,8(7):867-871.

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