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酶体外定向进化(Ⅱ)文库筛选的方法及其应用

DOI: 10.3969/j.issn.1000-5013.2005.02.001, PP. 113-116

Keywords: ,筛选,定向进化

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Abstract:

定向进化是改造蛋白质分子的一种有效的新策略,但其构建的文库非常大,待筛选的克隆常常多达105~1010.同时在目的酶筛选中,至关重要的是找到高灵敏度、高选择性和高通量的筛选方法.居于此,文中综合评述在突变株分离、酶检测和信号探测等方面,目前的一些常用技术及其最新进展.

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