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NaCS/PDMDAAC????????????????????1,3-??????

DOI: 10.11830/ISSN.1000-5013.2008.04.0554

Keywords: ?????????, ????????, ???????????, 1, 3-??????

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Abstract:

研究一种新型的生物微胶囊体系――NaCS/PDMDAAC,包埋肺炎克雷伯氏菌(K.pneumoniaeZJU5205)产1,3-丙二醇.比较不同初始菌体包埋量、胶囊/发酵液体积、发酵液pH值、初始甘油质量浓度等对微囊化细胞发酵结果的影响.结果表明,将细胞种子液稀释5倍后包埋,当胶囊与发酵液的体积比为1∶2、初始pH值为7、初始甘油质量浓度为60 g.L-1时,得到较好的发酵结果.考察肺炎克雷伯氏菌在囊内的生长曲线,以及底物和产物质量浓度随发酵时间的变化,发现由于胶囊引起的扩散限制,可以持续维持囊内较低的底物质量浓度,从而部分克服高质量浓度底物对菌体生长和生成产物的抑制.

References

[1]  ZENG A P, BIEBL H. Bulk chemicals from biotechnology:The case of 1, 3-propanediol production and the new trends [J]. Advances in Biochemical Engineering/Biotechnology, 2002.239-259.
[2]  SAINT-AMANS S, PERLIT P, GPMA G. High production of 1, 3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system [J]. Biotechnology Letters, 1994.832-836.
[3]  MENZEL K, ZENG A P, DECKWER W D. High concentration and productivity of 1, 3-propanediol from continuous fermentation of glycerol by Klebsiella pneumoniae [J]. Enzyme and Microbial Technology, 1997.82-86.
[4]  CHEN X, ZHANG D J, QI W T. Microbial fed-batch production of 1, 3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions [J]. Applied Microbiology and Biotechnology, 2003.143-146.
[5]  BIEBL H, MARTEN S. Fermentation of glycerol to 1, 3-propanediol:Use of cosubstrates [J]. Applied Microbiology and Biotechnology, 1995.15-19.
[6]  姚善泾. 新型生物微胶囊体系的生物相容性研究 [J]. 生物工程学报, 1998(2):193-197.
[7]  张俊, 姚善泾, 应小姣. 大孔型NaCS-PDMDAAC微胶囊固定化细胞在摇床和鼓泡塔中的培养研究 [J]. 生物工程学报, 2005(4):633-637.doi:10.3321/j.issn:1000-3061.2005.04.023.
[8]  王剑锋, 修志龙, 范圣第. 甘油转化生产1, 3-丙二醇发酵液中甘油含量的测定 [J]. 工业微生物, 2001(2):33-35.doi:10.3969/j.issn.1001-6678.2001.02.010.
[9]  MENZEL K, ZENG A P, BIEBL H. Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: I. The phenomena and characterization of oscillation and hysteresis [J]. Biotechnology and Bioengineering, 1996, (5):549-560.doi:10.1002/(SICI)1097-0290(19961205)52:5< 549::AID-BIT2> 3.0.CO; 2-J.
[10]  HOMANN T, TAG C, BIEBL H. Fermentation of glycerol to 1, 3-propanediol by Klebsiella and Citrobacter strains [J]. Applied Microbiology and Biotechnology, 1990.121-126.
[11]  刘德华, 向波涛, 刘宏娟. 1, 3-丙二醇发酵过程中底物抑制及其对策的研究 [J]. 现代化工, 2002(7):34-38.doi:10.3321/j.issn:0253-4320.2002.07.009.
[12]  ZENG A P, ROSS A, BIEBL H. Multiple product inhibition and growth modeling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation [J]. Biotechnology and Bioengineering, 1994.901-911.

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