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重组腺相关病毒质量控制的qPCR技术研究进展

DOI: 10.11830/ISSN.1000-5013.2014.02.0191

Keywords: 重组腺相关病毒, 基因组滴度, 实时荧光定量PCR, 基因治疗

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Abstract:

综述实时荧光定量PCR(qPCR)技术在重组腺相关病毒(rAAV)的基因组滴度测定中的应用成果,以及qPCR技术在rAAV质量控制过程中的应用,如感染滴度及rcAAV污染率的测定等.分析qPCR技术存在的适用范围窄、易产生系统性误差和易受rAAV杂质干扰等问题的原因,并探讨相关的解决策略.

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