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巨尾桉胞质EuCuZnSOD基因的克隆与原核表达

DOI: 10.11830/ISSN.1000-5013.2015.06.0693

Keywords: 巨尾桉, EuCuZnSOD基因, 铜锌超氧化物歧化酶, 克隆, 原核表达

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Abstract:

通过克隆巨尾桉的细胞质EuCuZnSOD基因,构建原核表达载体pET-CuZnSOD,并研究该基因的功能.测序结果表明:基因序列长度为459 bp;152个编码氨基酸,蛋白相对分子质量为15.2 ku.在28 ℃条件下,1 mmol?L-1 异丙基硫代半乳糖苷(IPTG)诱导4 h,转化菌株的超氧化物歧化酶(SOD),总酶活比对照组平均高19.9%.结果表明:克隆获得的巨尾桉细胞质EuCuZnSOD的重组基因表达产物具有SOD酶活性,GenBank注册号为JX138573.

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