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材料工程  2008 

碳纳米管与PVA协同加强光谱探针并应用于灵敏测定肝素钠

Keywords: 碳纳米管,乙基紫,光谱探针,肝素钠,协同加强

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Abstract:

提出将碳纳米管(CNTs)作为一种新型分析增效试剂用于加强光谱探针,研究了其与水溶性大分子增效试剂聚乙烯醇(PVA)的协同增敏机理,并应用于乙基紫(EV)光度法测定肝素钠(Heparin,Hep)的体系中。在此缔合物显色体系中,含芳香环大分子的EV通过π~π作用于CNTs的管壁上,增大了有色物质的吸光面积;PVA的加入提高了CNTs及离子缔合物的分散性。结果表明,在CNTs与PVA复配的影响下,EV与Hep在pH为6.5的Britton-Robinson(B-R)缓冲溶液中形成稳定的紫红色缔合物后在640nm处发生明显的褪色,ΔAmax提高了330.7%。肝素钠浓度在0~2.5mg/L范围内与ΔA成正比,回归方程为ΔA=-0.006+0.360C(mg/L),表观摩尔吸光系数ε=4.29×106L·mol-1·cm-1,R=0.9991。在最佳实验条件下,对肝素钠注射液效价进行了测定。

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