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同位素  2011 

特异性前哨淋巴结显像剂99Tcm-rituximab药盒的制备及生物评价

, PP. 85-89

Keywords: 99Tcm-rituximab,前哨淋巴结,显像剂

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Abstract:

采用2-巯基乙醇修饰Rituximab(利妥昔单克隆抗体),制得其冻干药盒。所制得的冻干药盒性质稳定,可在-20℃冷冻保存3个月以上。利用99Tcm-葡庚糖酸钠(GH)交换法,标记冻干药盒得到99Tcm-rituximab,标记率和放化纯度均大于90%。生物分布结果显示SD大鼠经前脚掌皮下注射99Tcm-rituximab后,前哨淋巴结的摄取值在1~4h内逐渐增加,在4h时达到(2.14±0.46)%ID,前哨淋巴结与注射点的放射性摄取比在4h时达到最大(14.80%±2.11%),且在18h时,这一比值基本保持不变。SPECT显像结果表明,在30min~18h内,大鼠的前哨淋巴结清晰可见,无次级淋巴结显影。本研究提供了一种特异性前哨淋巴结显像剂的药盒化制备方法,该方法操作简便,性能稳定,便于在临床推广应用。

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