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同位素  2012 

13C呼吸试剂D-木糖-U-13C5的合成

, PP. 209-213

Keywords: D-木糖-U-13C5,13C呼吸试剂,合成

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Abstract:

以D-葡萄糖-U-13C6为起始原料,采用减碳法经双丙酮保护、选择性酸水解、氧化、还原和碱水解五步合成D-木糖-U-13C5,总产率为38.6%(以D-葡萄糖-U-13C6计)。对每步产物使用相应的气相色谱(GC)、气质联用仪(GC-MS)、液相色谱(HPLC)和液质联用仪(LC-MS)进行表征。分析结果表明,每步反应中的产物均为目的产物,同位素丰度和纯度都符合要求,最后一步的产物为D-木糖-U-13C5,其13C丰度为99.1%,化学纯度为99.9%,符合13C呼吸试验的要求。

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