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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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~(125)I标记免疫球蛋白重链V1家族框架区反义寡核苷酸
, PP. 65-65
Keywords: 反义寡核苷酸,125I,标记
Abstract:
用DNA合成仪将含酪胺的单体连接在18聚体寡核苷酸的5′端,并用氯胺-T法进行125I标记。该标记方法的标记率为80.7%,标记物放化纯度为98.7%,体外放置24h放化纯度仍高于90%。聚丙烯酰胺凝胶电泳(PAGE)显示各时间点的条带与未保温的对照组平行,均未见异常条带。
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