|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
~(125)I标记免疫球蛋白重链V1家族框架区反义寡核苷酸
, PP. 65-65
Keywords: 反义寡核苷酸,125I,标记
Abstract:
用DNA合成仪将含酪胺的单体连接在18聚体寡核苷酸的5′端,并用氯胺-T法进行125I标记。该标记方法的标记率为80.7%,标记物放化纯度为98.7%,体外放置24h放化纯度仍高于90%。聚丙烯酰胺凝胶电泳(PAGE)显示各时间点的条带与未保温的对照组平行,均未见异常条带。
| [1] | 王荣福主编.核医学[M].北京:北京大学医学出版社,2003.71～72.
|
| [2] | PanyutinI,NeumannR.Sequence-specificDNABreaksProducedbyTriplex-directedDecayofIo-dine-125[J].ActaOncol,1996,35:817～823.
|
| [3] | CommerfordSL.IodinationofNucleicAcidsinVitro[J].Biochem,1971,10:1993～1999.
|
| [4] | DewanjeeMK,GhafouripourAK,KapadvanjwalaM,etal.NoninvasiveImagingofc-mycOnco-geneMessengerRNAWithIndium-111-antisenseProbesinAMammaryTumor-bearingMouseModel[J].JNuclMed,1994,35(6):1054～1063.
|
| [5] | 刘炳辰,岳井银,穆传杰,等.碘标记c-myc反义寡核苷酸乳腺癌显像的实验研究[J].中华核医学杂志,2000,20:33～34.
|
| [6] | LarsonSM,TjuvajevJ,BlasbergR.TriumphOverMischance:ARoleforNuclearMedicineinGeneTherapy[J].JNuclMed,1997,38(8):1230～1233.
|
| [7] | WangRF,QiuF,ZhangCL.LabelingofAnti-senseOligonucleotidesWith125IandtheExperi-mentalResearchofIdentificationoftheLym-phomaCellbyUsingI-125-FR-ASON(Abstract)[J].JNuclMed,2003,44(5suppl):369.
|
| [8] | TavitianB.InVivoAntisenseImaging[J].QJNuclMed,2000,44:236～255.
|
| [9] | CammilleriS,SangrajrangS,PerdereauB,etal.BiodistributionofIodine-125TyramineTrans-formingGrowthFactorAlphaAntisenseOligonu-cleotideinAthymicMiceWithaHumanMamma-ryTumourXenograftFollowingIntratumoralIn-jection[J].EurJNuclMed,1996,23(4):448～452.
|
| [10] | 邱峰,王荣福,张春丽,等.125I标记反义寡核苷酸及其识别淋巴瘤细胞的实验研究[J].中华核医学杂志,2003,23(2):69～71.
|
| [11] | PanyutinI,NeumannR.Sequence-specificDNABreaksProducedbyTriplex-directedDecayofIo-dine-125[J].ActaOncol,1996,35:817～823.
|
| [12] | HanotowichDJ.AntisenseandNuclearMedicine[J].JNuclMed,1999,40:693～703.
|
| [13] | 郑建国,谭天秩,匡安仁,等.脂质体介导的
|
| [14] | 99Tcm标记c-mycmRNA反义寡核苷酸初步研究[J].同位素,2001,14(2):88～91.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
