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去甲肾上腺素聚合膜固定葡萄糖氧化酶用于葡萄糖传感

, PP. 372-376

Keywords: 去甲肾上腺素,葡萄糖氧化酶,聚合,传感

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Abstract:

研究了中性缓冲溶液中葡萄糖氧化酶在去甲肾上腺素循环伏安电氧化聚合膜中的共沉积.考察了共沉积时去甲肾上腺素和葡萄糖氧化酶浓度的影响,结果表明5.5mmol/L去甲肾上腺素(NA)和4.0mg/mL葡萄糖氧化酶(GOD)时所制葡萄糖传感器性能最佳.也考察了检测电位、溶液pH及电活性物质干扰等因素.当溶液pH值为7.0,检测电位为0.7V时,传感器响应电流与葡萄糖浓度在0.059.1mmol/L之间呈现良好的线性关系,检测下限为2μmol/L(S/N=3),且传感器的响应时间短(15s内)、抗干扰能力强、稳定性好.测得固定酶的米氏常数(Kappm)为11.3mmol/L.

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