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甘蓝型油菜FAE1基因启动子的克隆和瞬时表达载体的构建及功能分析

, PP. 719-723

Keywords: FAE1启动子,瞬时表达,荧光素酶

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Abstract:

油菜脂肪酸延长酶基因FAE1是广泛存在于植物中并定位于内质网上的一种能催化脂肪酸碳链延长的酮脂酰CoA合成酶,根据GenBank上已知的植物FAE1基因启动子序列设计引物,以从甘蓝型油菜(Braccicanapus.L)叶片中提取的总DNA为模板,进行PCR扩增,获得了1328bp的片段.回收该片段,并连接到pMD18-T载体测序.序列在NCBI进行blast比对,同源性为99.78%,说明该片段与植物中已知的FAE1基因的启动子序列有极高的相似性.将该片段替换掉改造后的pBI221上面的35S启动子从而构建了原生质体瞬时表达载体.然后通过PEG介导的方式将含有FAE1启动子驱动的荧光素酶报告基因(LUC)表达载体导入油菜原生质体中,研究报告基因表达量,证明出脱落酸(ABA)影响了报告基因的表达,随着ABA浓度的不断增加,LUC的表达量呈现逐步下降的趋势.

References

[1]  James Jr D W, Lim E, Keller J, et al. Directed tagging of the arabidopsis fatty acid elongation(FAE1) gene with the maize transposon activator K[J]. Plant Cell,1995,7(3):309-319.
[2]  Anthony A M, Mercedes W, Ljerka K. Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic aAlterations in plant morphology[J]. Plant Cell,1998,10:1889-1902.
[3]  Giuliana G, Chiara T, Roberto M. Regulation of novel members of the arabidopsis thaliana CCAAT-binding nuclear factor Y subunits[J]. Gene,2002,283:41-53.
[4]  李一琨,王金发. 高等植物启动子研究进展[J]. 植物学通报,1998,15(S):1.
[5]  王颖,麦维军,梁承晔,等. 高等植物启动子的研究进展[J]. 西北植物学报,2003,23(11):2040.
[6]  Xu D P, David M, Robert W T, et al. Systemic induction of a potato pin2 promoter by wounding, methyl iasmonate, and abscisic acid in transgene rice plants[J]. Plant Molecular Biology,1993,22:573.
[7]  Christmann A, Moes D, Himmelbach A, et al. Integration of abscisic acid sigaling into plant responses[J]. Plant Bio,2006,8:314-325.
[8]  Yang Y, Sulpice R, Himmelbach A, et al. Fribrillin accumulation is regulated by ABI2 and is involved in abscisc acid-mediated photoprotection[J]. PNAS,2006,103:6061.
[9]  刘惠丽,李玲. 脱落酸(ABA)诱导基因表达的调控元件[J]. 植物学通报,2001,18(3):276.
[10]  lubberstedt T, Oelmuller R, Wanner G, et al. Interacting cis elements in the plastocyanin promoter from spinach ensure regulated high-level expression[J]. Molecular and General Genetics,1994,242(5):1.
[11]  陈章良,瞿礼嘉. 植物基因的调控机制探讨[J]. 生物工程进展,1991,11(4):8.

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