Borges O, Silva M, Sousa A D, et al. Alginate coated chitosan nanoparticles are an effective subcutaneous adjuvant for hepatitis B surface antigen\[J\]. International Immunopharmacology, 2008,8(13/14):1773-1780.
[2]
Hamidi M, Azadi A, Rafiei P. Hydrogel nanoparticles in drug delivery\[J\]. Advanced Drug Delivery Reviews,2008,60(15):1638-1649.
[3]
George M, Abraham T E. pH sensitive alginate-guar gum hydrogel for the controlled delivery of protein drugs\[J\]. International J Pharmaceutics,2007,335(1/2):123-129.
[4]
Yuan Y, Chesnutt B M, Utturkar G, et al. The effect of cross-linking of chitosan microspheres with genipin on protein release\[J\]. Carbohydrate Polymers,2007,68(3/5):561-567.
Study on the Impact of Fluorescence Labeling Reaction of Chitosancalcium
[9]
Alginate Microsphere on Microsphere Membrane Strength
[10]
FAN Ni1,XUE Weiming2
[11]
(1. Department of Chemistry and Chemical, Shaanxi Institute of Education, Xi’an 710100, Shaanxi;
[12]
2. College of Chemical Engineering, Northwest University, Xi’an 710061, Shaanxi)Abstract:This paper investigates the impact of fluorescence labeling reaction of chitosancalcium alginate microsphere for labeling on microphere membrane strength in simulated body liquid under different conditions in order to detect such microspheres in vivo. With chitosan and sodium alginate as materials for preparing microsphere and with isothiocyanate FITC as fluorescence label for labeling microspheres, the rate of expansion of FITClabeled chitosanalginate microspheres is determined to characterize microsphere membrane strength. The FITClabeled microspheres are prepared with the FITClabeled chitosan of which concentration is 0.015 g/mL reacting 30 min with calcium alginate microspheres. On the other hand, chitosan molecular weight is 50 000, the degree of deacytylation is 85% and the solution concentration is 0.01 g/mL. Such FITClabeled microspheres show better membrane strength and stability. The appropriate experimental conditions are provided to study the distribution、absorbency and biodegradation characteristics of drugloaded chitosanalginate microsphere in vivo.Key words:chitosan; calcium alginate; microsphere; fluorescencelabeled; membrane strength(编辑余毅)
