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基于L-半胱氨酸/纳米金固定酶的过氧化氢生物传感器

, PP. 236-239

Keywords: L-半胱氨酸,纳米金,过氧化物酶,生物传感器,层层自组装

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Abstract:

将L-半胱氨酸电聚合于金电极表面,通过静电作用和共价结合,吸附带负电荷的纳米金(nano-Au),最后再利用纳米金吸附固定过氧化物酶(HRP),制备了一种新型层层自组装的电流型过氧化氢(H2O2)传感器.采用交流阻抗和计时电流法对该传感器的性能进行了详细研究.实验表明,该传感器增加了酶的吸附量,响应快、稳定性好,对H2O2表现出良好的响应特性.在0.1mol/LPBS(pH值6.5)缓冲溶液中,电位为0.3V的实验条件下,该传感器对H2O2检测范围为3.5×10-6~5.9×10-3mol/L,检

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