全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Sorex bedfordiae的性比和Soricidea动物性别的分子鉴定

, PP. 280-283

Keywords: 小纹背鼩鼱,鼩鼱科,性别鉴定,性比,Sry基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

以小纹背鼩鼱(Sorexbedfordiae)幼体和外部性别鉴别特征缺失的小纹背鼩鼱个体为材料,用Sry基因的一对性别鉴定引物成功鉴定了82只未知性别的小纹背鼩鼱的性别,最终确定了355只小纹背鼩鼱种群的性比,雌性199只,雄性156只.此外,用鼩形目中的11个物种检测了该对引物的实用性,结果表明该引物适用于鼩鼱科中的大纹背鼩鼱(Sorexcylindricauda)、高原鼩鼱(Sorexexcelsus)、中鼩鼱(Sorexcaecutiens)、黑齿鼩鼱(Blarinellaquadraticauda)、灰黑齿鼩鼱(Blarinellagriselda)、狭颅黑齿鼩鼱(Blarinellawardi)和灰麝鼩(Crociduraattenuata)7个物种.研究结果为鼩鼱科动物的性别鉴定提供了快速准确的方法.

References

[1]  Grainger J P, Fairle J S. Studies on the biology of the pygmy shrew Sovex minutus in the west of Ireland[J]. J Zoology,1978,186:109-141.
[2]  Blair W F. Notes on home ranges and populations of the short-tailed shrew[J]. Ecology,1940,21:284-288.
[3]  Rudd R L. Age, sex and weight comparisons in three species of shrews[J]. J Mammal,1955,36:323-339.
[4]  Middleton A D. A contribution to the biology of the common shrew, Sorex araneus, Linnaeus[J]. Proc Zoological Soc London,1931,101:133-143.
[5]  Sinclair A H, Berta P, Palmer M, et al. A gene from the human sex determining region encodes a protein with homology to a conserved DNA-binding motif[J]. Nature,1990,346:240-244.
[6]  Sánchez A, Bullejos M, Burgos M, et al. High sequence identity between the Sry HMG box from humans and insectivores[J]. Mammalian Genome,1996,7:536-538.
[7]  Bryja J, Konecny A. Fast sex identification in wild mammals using PCR amplification of the Sry gene[J]. Folia Zoologica,2003,52:269-274.
[8]  Matsubara K, Ishibashi Y, Ohdachi S, et al. A new primer set for sex identification in the genus Sorex(Soricidae, Insectivora)[J]. Molecular Ecology Notes,2001,1:241-242.
[9]  王应祥. 中国哺乳动物种和亚种分类名录与分布大全[M]. 北京:中国林业出版社,2003.
[10]  Smith A T,解焱. 中国兽类野外手册[M]. 长沙:湖南教育出版社,2009.
[11]  刘洋,孙治宇,王昊,等. 西藏小型兽类五新纪录[J]. 四川动物,2009,28(2):278-279.
[12]  陈顺德. 中国鼩鼱亚科(Soricidae)部分类群的分类和生物地理研究[D]. 成都:四川大学图书馆,2012.
[13]  Sambrook E, Fritsch F, Maniatis T. Molecular Cloning: A Laboratory Manual[M]. New York:Cold Spring Harbour Laboratory Press,1989.
[14]  Irwin D M, Kocher T D, Wilson A C. Evolution of the cytochrome b gene of mammals[J]. J Molecular Evolution,1991,2:128-144.
[15]  Pruitt W O. Notes on the short tail shrew (Blarina brevicauda kirtlandi) in northern Lower Michigan[J]. Am Midland Nat,1954,52:236-241.
[16]  蒋凡,徐翔,罗林明,等. 四川短尾鼩生物学研究[J]. 西南农业大学学报:自然科学版,1999,21(5):460-464.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133