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EGF通过PI3K/AKT通路介导小鼠卵巢颗粒细胞增殖

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Abstract:

目的探讨表皮生长因子(EGF)介导,经PI3K/AKT信号通路调控小鼠卵巢颗粒细胞增殖的作用与机制。方法取分离纯化的小鼠卵巢颗粒细胞,添加10ng?mL-1的EGF对其培养24h后,然后再添加20μM浓度的P13K抑制剂LY294002处理细胞72h,利用CCK-8检测颗粒细胞增殖情况;应用Real-timePCR法检测AKTmRNA的表达;应用Westernblot法检测体外培养小鼠颗粒细胞中总AKT(t-AKT)及磷酸化AKTThr308位点(p-AKTThr308)蛋白的表达情况。结果(1)10ng?mL-1的EGF对小鼠颗粒细胞增殖效应明显,能够显著提高AKT基因在颗粒细胞中的表达(P<0.05),能显著性促进t-AKT及p-AKTThr308蛋白的表达(P<0.05)。(2)PI3K特异性抑制剂LY294002明显抑制EGF促进颗粒细胞增殖的效应(P<0.05)。结论EGF能活化小鼠颗粒细胞的PI3K/AKT信号通路,促进颗粒细胞生长、增殖。

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