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荷包牡丹碱和NMDA对大鼠布比卡因蛛网膜下腔阻滞作用的影响

, PP. 375-378

Keywords: 蛛网膜下腔,荷包牡丹碱,N-甲基-D-天冬氨酸,丙泊酚,镇静

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Abstract:

目的观察蛛网膜下腔注射荷包牡丹碱和N-甲基-D-天冬氨酸(N-Methyl-D-Aspartate,NMDA)对大鼠丙泊酚镇静剂量的影响。方法雄性SD大鼠40只,随机分为5组空白对照组(C组)、生理盐水组(NS组)、0.5%布比卡因组(B组)、荷包牡丹碱组+0.5%布比卡因(Bic组)、NMDA+0.5%布比卡因组(NMDA组),每组8只。建立大鼠蛛网膜下腔阻滞模型,NS组和B组分别蛛网膜下腔注射20μL生理盐水和0.5%布比卡因;Bic组和NMDA组蛛网膜下腔分别注射10μL有效剂量荷包牡丹碱和NMDA,15min后蛛网膜下腔注射0.5%布比卡因20μL;5组大鼠均于蛛网膜下腔给药后10min行尾静脉注射丙泊酚,比较组间大鼠眼睑反射消失时丙泊酚的用量及所需时间。结果B组大鼠眼睑反射消失时的丙泊酚用量(6.72±0.77)mg?kg-1明显少于C组(10.94±0.91)mg?kg-1和NS组(10.51±1.01)(P0.05)。结论布比卡因蛛网膜下腔阻滞可以减少大鼠丙泊酚的镇静用量;大鼠蛛网膜下腔阻滞产生的镇静作用可能与脊髓内的GABAA受体有关,而与NMDA受体无明显关系。

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