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生物信息学技术在结核分支杆菌ESAT-6 重组抗原研究中的应用

, PP. 334-336

Keywords: 生物信息学,结核分支杆菌,ESAT-6,重组抗原

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Abstract:

目的应用生物信息学分析软件预测分析结核分支杆菌ESAT-6基因及相关蛋白的特性。方法应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析ESAT-6并进行同源比对;预测二级、三级结构,以及预测主要抗原表位等。结果结核分支杆菌重组抗原ESAT-6与已发表氨基酸序列同源性为90%。预测该蛋白分子质量约为9885.7Da,PI为4.6,4个抗原表位,其结构域位于56-87位。结论生物信息学技术在结核分支杆菌ESAT-6重组抗原研究中有一定的理论和应用价值。

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