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RUNX3、DNMT1 mRNA在原发性肝细胞癌中的表达及其意义

Keywords: 原发性肝细胞癌,RUNX3,DNMT1,逆转录聚合酶链反应

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Abstract:

目的研究人RUNT相关转录因子3(Runt-relatedtranscriptionfactor3,RUNX3)和DNA甲基转移酶1(DNAmethyltransferase1,DNMT1)在原发性肝细胞癌(primaryhepatocellularcarcinoma,HCC)中的表达及其与临床病理学特征的关系,并探讨其临床意义。方法应用逆转录聚合酶链反应(Reversetranscription-Polymerasechainreaction,RT-PCR)检测43例HCC患者癌组织及其相应癌旁组织中RUNX3mRNA、DNMT1mRNA的表达。结果肝癌组织中RUNX3表达明显降低,DNMT1表达增高;RUNX3表达下调与组织分化程度有关(?P?<0?05),而与性别、年龄、HBsAg、AFP、静脉癌栓及TNM分期均无明显关系(?P?>0?05);DNMT1表达升高与静脉癌栓、组织分化程度及TNM分期有关(?P?<0?05),而与性别、年龄、HBsAg、AFP均无明显关系(?P?>0?05)。RUNX3与DNMT1表达成负相关(?r?=-0?2059,?P?<0?05)。结论RUNX3基因在肝癌组织中表达下调,DNMT1表达增高,可能与肝癌的发生发展有关;高表达的DNMT1在一定程度上参与调控RUNX3基因的表达下调。

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