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口腔上皮细胞感染白假丝酵母菌后HBD-2蛋白的表达

, PP. 1160-1163

Keywords: 口腔上皮细胞,白假丝酵母菌,人β防御素2

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Abstract:

目的检测人β防御素2(Humanbetadefensin-2,HBD-2)在口腔上皮细胞中的表达以及白假丝酵母菌对其表达的影响。方法分别以孢子相、菌丝相以及灭活的白假丝酵母菌与KB细胞共同培养6、12、24h后,采用ELISA技术检测各组KB细胞培养液中HBD-2蛋白的表达情况,与对照组进行比较。结果KB细胞有HBD-2蛋白的基础表达,感染6h后,各实验组与对照组之间表达差异无统计学意义(P>0.05);感染12h后,孢子组(93.974±6.426)、菌丝组(72.287±2.839)显著高于对照组(37.380±5.663),P<0.01;感染24h后,各实验组表达均高于对照组(P<0.05),但和12h时的表达相比,孢子组(80.851±7.860)、菌丝组(63.932±8.070)出现了下降趋势(P<0.05)。结论HBD-2在口腔上皮细胞中有基础表达,白假丝酵母菌能诱导其表达上调;白假丝酵母菌对HBD-2的诱导表达只发生在感染的特定时期内,当菌丝进一步侵袭后,HBD-2的表达会逐渐减弱。

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