全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

热应激处理对精原细胞增殖的影响

, PP. 1131-1134

Keywords: 热应激,精原细胞,增殖,干细胞微环境

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的了解热应激对小鼠精原细胞增殖情况的影响,探讨高温造成男性短期不育的机制。方法8周龄雄性昆明白小鼠于43℃水浴中进行睾丸局部热应激处理15min,于一次性处理后1、12、18、25d以及35d通过PCNA免疫荧光组化法检测精原细胞增殖情况;于一次性处理后4、2、10、15、20d以及25d通过RT-PCR检测GDNF和SCF基因表达情况。结果在热处理后第12d和18d,精原细胞增殖系数分别为22%和75%,明显低于(P<0.05)对照组的93%;25d时,精原细胞增殖水平恢复至正常水平。GDNFmRNA的表达量在处理后4h到第15d显著下调(P<0.05);SCFmRNA的表达量在热处理后第4h到第10天显著下调(P<0.05)。结论睾丸局部热应激处理使得GDNF和SCF基因表达在处理后短时间内下调,精原细胞增殖短时间内抑制。

References

[1]  Zhang Y, Yang X, Cao H, et al. Heat stress induces Cdc2 protein decrease prior to mouse spermatogenic cell apoptosis[J]. Acta Histochem, 2008, 110(4):276-284.
[2]  Schmidt JA, Avarbock MR, Tobias JW, et al. Identification of glial cell line-derived neurotrophic factor-regulated genes important for spermatogonial stem cell self-renewal in the rat[J]. Biol Reprod, 2009, 81:56-66.
[3]  Unni SK, Modi DN, Pathak SG, et al. Stage-specific localization and expression of c-kit in the adult human testis[J]. J Histochem Cytochem, 2009, 57:861-869.
[4]  de Rooij DG. The spermatogonial stem cell niche[J]. Microsc Res Tech, 2009, 72:580-585.
[5]  Weise JM, Gunes C. Differential regulation of human and mouse telomerase reverse transcriptase(TERT)promoter activity during testis development[J]. Mol Reprod Dev 2009, 76:309-317.
[6]  Lue YH, Hikim AP, Swerdloff RS, et al. Single exposure to heat induces stage-specific germ cell apoptosis in rats: role of intratesticular testosterone on stage specificity[J]. Endocrinology, 1999, 140(4):1709-1717.
[7]  Creemers LB, Meng X, den Ouden K, et al. Transplantation of germ cells from glial cell line-derived neurotrophic factor-overexpressing mice to host testes depleted of endogenous spermatogenesis by fractionated irradiation[J]. Biol Reprod, 2002, 66(6):1579-1584.
[8]  Meng X, Lindahl M, Hyvonen ME, et al.Regulation of cell fate decision of undifferentiated spermatogonia by GDNF[J]. Science, 2000, 287(5457):1489-1493.
[9]  Hofmann MC, Braydich-Stolle L, Dym M. Isolation of male germ-line stem cells; influence of GDNF[J]. Dev Biol,2005, 279(1):114-124.
[10]  Viglietto G, Dolci S, Bruni P, et al. Glial cell line-derived neutrotrophic factor and neurturin can act as paracrine growth factors stimulating DNA synthesis of Ret-expressing spermatogonia[J]. Int J Oncol, 2000, 16(4):689-694.
[11]  Ohta H, Yomogida K, Dohmae K, et al. Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF[J]. Development, 2000, 127(10):2125-2131.
[12]  Danno S, Itoh K, Matsuda T, Fujita J. Decreased expression of mouse Rbm3, a cold-shock protein, in Sertoli cells of cryptorchid testis[J]. Am J Pathol, 2000, 156(5):1685-1692.
[13]  Jannes P, Spiessens C, Van der Auwera I, et al. Male subfertility induced by acute scrotal heating affects embryo quality in normal female mice[J]. Hum Reprod,1998, 13(2):372-375.
[14]  Setchell BP, Ekpe G, Zupp JL, et al. Transient retardation in embryo growth in normal female mice made pregnant by males whose testes had been heated[J]. Hum Reprod, 1998, 13(2):342-347.
[15]  Setchell BP, D'Occhio MJ, Hall MJ, et al. Is embryonic mortality increased in normal female rats mated to subfertile males[J].J Reprod Fertil,1988, 82(2):567-574.
[16]  Rockett JC, Mapp FL, Garges JB, et al. Effects of hyperthermia on spermatogenesis, apoptosis, gene expression, and fertility in adult male mice[J]. Biol Reprod, 2001, 65(1):229-239.
[17]  de Rooij DG: Stem cells in the testis[J]. Int J Exp Pathol, 1998, 79(2):67-80.
[18]  Naughton CK, Jain S,Strickland AM, et al.Glial cell-line derived neurotrophic factor-mediated RET signaling regulates spermatogonial stem cell fate[J]. Biol Reprod, 2006, 74(2):314-321.
[19]  Chowdhury AK, Steinberger E. A Quantitative Study of the Effect of Heat on Germinal Epithelium of Rat Testes[J]. Am J Anat, 1964, 115:509-524.
[20]  Zhang XS, Zhang ZH, Jin X, et al. Dedifferentiation of adult monkey Sertoli cells through activation of extracellularly regulated kinase 1/2 induced by heat treatment[J]. Endocrinology,2006, 147(3):1237-1245.
[21]  Kubota H, Avarbock MR, Brinster RL. Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells[J]. Biol Reprod, 2004, 71(3):722-731.
[22]  Kubota H, Avarbock MR, Brinster RL. Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells[J]. Proc Natl Acad Sci USA, 2004, 101(47):16489-16494.
[23]  Tadokoro Y, Yomogida K, Ohta H, et al. Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway[J]. Mech Dev,2002, 113(1):29-39.
[24]  Yomogida K, Yagura Y, Tadokoro Y, et al. Dramatic expansion of germinal stem cells by ectopically expressed human glial cell line-derived neurotrophic factor in mouse Sertoli cells[J]. Biol Reprod, 2003, 69(4):1303-1307.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133