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氧化苦参碱对肝癌细胞QGY-7703迁移侵袭?能力和MMP-9表达的影响

, PP. 1243-1245

Keywords: 氧化苦参碱,肝癌细胞,细胞迁移,细胞侵袭,MMP-9

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Abstract:

目的通过观察氧化苦参碱(oxymatrine,OM)对人肝癌细胞QGY-7703体外迁移侵袭能力和基质金属蛋白酶-9(matrixmetalloproteinase9,MMP-9)表达的影响,初步探讨氧化苦参碱对肝癌细胞迁移侵袭能力的影响及可能的分子机制。方法体外培养人肝癌细胞QGY-7703并用OM干预后,Transwell迁移和侵袭实验检测OM对细胞迁移侵袭能力的影响;定量PCR检测细胞中MMP-9mRNA的表达水平,WesternBlot检测细胞中MMP-9蛋白的表达水平。结果与细胞对照组相比,低浓度(1mg?mL?-1)OM干预组QGY-7703细胞的迁移侵袭能力未发生显著变化;中浓度(2.5mg?mL?-1)和高浓度(5mg?mL?-1)OM干预组细胞的迁移侵袭能力受到明显抑制(P<0.01),同时伴有MMP-9mRNA和蛋白表达水平的下降(P<0.01)。结论OM在体外可抑制人肝癌细胞QGY-7703的迁移侵袭能力,并能抑制MMP-9的表达,提示OM可能通过抑制MMP-9的表达参与抗肿瘤细胞迁移侵袭的作用。

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