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慢性不可预测性温和应激对大鼠行为及海马微管蛋白表达的影响

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Keywords: 慢性不可预测性温和应激,海马,细胞骨架,微管蛋白

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Abstract:

目的研究慢性不可预测性温和应激对大鼠行为及海马微管蛋白表达的影响。方法将SD雄性大鼠24只随机分为对照组、慢性不可预测性温、应激组(chronicunpredictedmildstress,CUMS组)和氟西汀组,每组8只。在实验结束后对三组大鼠进行行为学观察,检测海马微管蛋白的表达。结果CUMS组大鼠总行程、平均运动速度、直立次数及糖水偏好低于对照组(P0.05)。免疫印迹结果显示CUMS组海马乙酰化微管蛋白(Acet-Tub)表达升高,酪氨酸化微管蛋白(Tyr-Tub)表达减低,与对照组比较差异有统计学意义(P<0.01);经氟西汀处理后,Acet-Tub表达降低,Tyr-Tub表达升高,与CUMS组比较差异有统计学意义(P<0.01)。结论慢性不可预测性温和应激能降低大鼠海马微管系统的动态性,损害神经可塑性,氟西汀可以逆转损害的微管结构,可能是抑郁样行为的分子机制。

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