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丙泊酚对发育期大鼠皮质神经元钙离子的影响

, PP. 1317-1320

Keywords: 丙泊酚,发育期,神经元,钙离子

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Abstract:

目的观察不同浓度丙泊酚对大鼠发育期大脑皮质神经元钙离子(Ca2+i)的影响。方法原代培养7d的大鼠皮质神经元,随机分为4组对照组(C组)、丙泊酚10μmol?L-1组(P10组)、丙泊酚30μmol?L-1组(P30组)、丙泊酚100μmol?L-1组(P100组),Fluo-4AM钙荧光指示剂染色,激光共聚焦显微镜下动态观察使用丙泊酚前、丙泊酚预处理后和氯化钾溶液诱发细胞内钙离子浓度的变化。结果培养7d的大鼠各组皮质神经元基础值、丙泊酚预处理各组钙荧光强度间比较差异无统计学意义(P>0.05);丙泊酚预处理后钙荧光强度同基础值比较差异无统计学意义(P>0.05);氯化钾诱发后各组钙荧光强度明显高于基础值(P0.05)。结论丙泊酚抑制大鼠发育期皮质神经元钙内流。

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