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氯沙坦钾对STZ诱导糖尿病大鼠肾小管间质纤维化的保护作用

, PP. 1336-1340

Keywords: 糖尿病肾脏疾病,肾小管间质纤维化,单核细胞趋化蛋白-1,α-平滑肌肌动蛋白

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Abstract:

目的观察氯沙坦钾对糖尿病大鼠肾小管间质纤维化中的保护作用。方法STZ诱导糖尿病大鼠模型。随机分为正常对照组(NC组),糖尿病模型组(DM组),氯沙坦钾干预组(ARB组)10.4mg?(kg?d)-1,每组10只。16周后,监测糖尿病大鼠肾功能、尿微量白蛋白、尿N-乙酰β-D氨基葡萄糖苷酶(NAG)、肾小管间质病理改变、及肾脏单核细胞趋化蛋白-1(MCP-1),α-平滑肌肌动蛋白(α-SMA)的表达。结果ARB组与DM组比较,血肌酐、尿素氮、尿微量白蛋白及NAG水平均下降(P均<0.05);DM组及ARB组大鼠肾小管间质损伤评分均高于NC组(P<0.01);ARB评分低于DM组(P<0.05)。ARB组与DM组相比肾小管间质MCP-1、α-SMA的mRNA表达(P<0.05)及免疫组织化学染色表达显著降低。结论氯沙坦钾可以通过抑制肾小管间质的炎性细胞浸润及上皮细胞的转分化发挥保护糖尿病大鼠肾脏的作用。

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