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表达口蹄疫病毒VP1的重组腺病毒对小鼠特异性免疫的影响

Keywords: 口蹄疫病毒,重组腺病毒,VP1基因

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Abstract:

目的检测表达FMDVVP1基因的重组腺病毒对小鼠特异性免疫的影响。方法6周龄雌性BALB/c小鼠随机分为3组,每组12只,将重组腺病毒rAd5VP1以腹膜内接种的方式免疫小鼠,对照组小鼠分别腹膜内接种等量的PBS和口蹄疫病毒灭活疫苗。通过测定IgG、IgG1、IgG2a、淋巴细胞增殖功能、IL-4和IFN-γ的水平来衡量重组病毒的免疫效果。结果重组腺病毒免疫小鼠既能刺激小鼠淋巴细胞增殖又能诱导小鼠特异性抗体的产生,证明重组病毒具有良好的免疫原性。免疫原性略低于灭活疫苗免疫组,但明显高于PBS组。结论重组病毒刺激小鼠淋巴细胞增殖和抗体分泌的能力略低于灭活疫苗,但重组病毒诱导Th1分化的能力强于灭活疫苗。

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