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槐定碱对内毒素肺损伤小鼠p38MAPK的影响

, PP. 1006-1009

Keywords: 槐定碱,内毒素,急性肺损伤,p38MAPK

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Abstract:

目的探讨内毒素(细菌脂多糖,LPS)肺损伤小鼠肺组织p38丝裂原活化蛋白激酶(p38MAPK)表达的变化以及槐定碱对其影响。方法60只BALB/c小鼠,除正常对照组、槐定碱对照组外,内毒素肺损伤模型组(LPS模型组)、槐定碱治疗高(12mg?kg-1)、中(6mg?kg-1)、低(3mg?kg-1)剂量组的BALB/c小鼠均尾静脉注射LPS制备急性肺损伤模型,注射后30min再分别腹腔注射生理盐水或槐定碱12、6、3mg?kg-1,各组均处理后6h取材,肉眼及光镜观察肺组织的病理变化,免疫组化SP法检测肺组织中总p38、磷酸化p38的表达,硝酸还原酶法检测血清NO含量。结果LPS模型组肺脏病理改变明显加重,肺组织总P38和磷酸化p38的表达均增多和增强(P<0.01);血清NO显著升高(P<0.01);槐定碱三个剂量治疗组病理损伤均不同程度改善,总P38表达与LPS组比较差异无统计学意义,但磷酸化p38表达均显著小于模型组(P<0.01),血清NO含量显著低于LPS模型组(P<0.01)。结论p38MAPK参与了内毒素肺损伤的发病过程,槐定碱的抗内毒素肺损伤机制可能与下调p38MAPK的表达,抑制NO的释放有关。

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