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槐定碱对小鼠巨噬细胞TLR4及JNK/c-jun mRNA表达的影响

Keywords: 槐定碱,脂多糖,TLR4,JNK,c-jun

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Abstract:

目的观察槐定碱预处理对LPS刺激RAW264.7巨噬细胞表达TLR4、JNK和c-junmRNA及分泌TNF-α的影响,探讨槐定碱抗内毒素机制。方法培养RAW264.7巨噬细胞,实验分为4组空白对照组、槐定碱对照组、LPS组、槐定碱预处理组,用RT-PCR技术检测RAW264.7细胞TLR4、JNK和c-jun的mRNA表达量,放免法检测细胞培养液TNF-α分泌量。结果槐定碱对RAW264.7巨噬细胞TLR4、JNK、c-jun的mRNA及细胞培养液中TNF-α基础表达无影响,但对经LPS激活的巨噬细胞的TLR4、JNKmRNA表达有显著抑制作用(均P<0.01),c-junmRNA表达亦降低,但与LPS模型组比较差异尚无统计学意义。槐定碱预处理组细胞液中TNF-α含量显著低于LPS模型组(P<0.01)。结论槐定碱抗内毒素机制与其抑制LPS引起的TLR4、JNK的mRNA高表达,并减少TNF-α分泌有关。

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