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生长抑素受体特异性激动剂 对人肝癌细胞生长影响的实验研究

, PP. 126-129

Keywords: 生长抑素受体亚型特异性激动剂,肝癌细胞

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Abstract:

目的探讨生长抑素受体特异性激动剂对体外培养的人肝癌细胞HepG2生长的影响。方法分别采用MTS、TUNEL、Transwell法检测生长抑素受体亚型特异性激动剂对体外培养的人肝癌细胞株HepG2增殖、凋亡、迁移的影响。结果与对照组相比,SSTR2~4亚型特异性激动剂对人肝癌细胞的增殖、凋亡、迁移没有明显影响,SSTR5亚型特异性激动剂L-817、818在剂量为1μg?mL-1时可以出现较明显的迁移抑制作用,剂量达到5μg?mL-1时,差异有统计学意义(P<0.05)。其他不同浓度激动剂对HepG2的迁移抑制作用不明显。结论SSTR亚型特异性激动剂对肝癌细胞的增殖、凋亡、侵袭没有明显的影响,L-817、818在高剂量时可以抑制HepG2的迁移。

References

[1]  Dimitroulopoulos D, Xinopoulos D, Tsamakidis K, et al. The role of somatostatinLAR in treating patients with advanced hepatocellular cancer[J]. Hepatogastroenterology, 2002,49:1245-1250.
[2]  Yuen MF, Poon RT, Lai CL, et al. A randomized placebo-controlled study of long-acting octreotide for the treatment of advanced hepatocellular carcinoma[J]. Hepatology, 2002,36:687-691.
[3]  Tomassetti P, Migliori M, Corinaldesi R, Gullo L. Treatment of gastroenteropa-ncreatic neuroendocrine tumours with octreotide LAR[J]. Aliment Pharmacol Ther, 2000,14:557-560.
[4]  Reynaert H, Rombouts K, Vandermonde A, et al. Expression of somatostatin receptors in normal and cirrhotic human liver and in hepatocellular carcinoma[J]. Gut, 2004,53(8): 1180-1189.
[5]  Guillermet-Guibert J, LahlouH, CordelierP, et al. Physiology of somatostatin receptors[J]. Endocrinol Invest, 2005, 28(11): 5-9.
[6]  刘欣,王宝成.SSTR2A与SSTR5及VEGF在非小细胞肺癌组织中的表达及与预后的关系[J].现代肿瘤医学,2008,16(6):955-958.
[7]  Yusuke Taniyama, Takashi Suzuki Yoshiki Mikami. Systemic dis-tribution of somatostatin receptor subtypes in human: An immuno-histochemical study[J]. Endocrine Journal, 2005,52(5):605-611.
[8]  Dimitroulopoulos D,Xinopoulos D,Tsamakidisk,et al.Long acting octreotide in the treatment of advanced hepatocellular cancer and overexpression of somatostatin receptors:randomized placebo-controlled trial[J].World JGastroenterol,2007,13(23):3164-3170.
[9]  武曼群,杨少奇,李昭宇,等.生长抑素受体亚型在人原发性肝癌中的表达及意义[J].宁夏医学院学报,2008,30(2):106-107.
[10]  王春晖,唐承薇,汤丽平.奥曲肽抑制肝癌生长的实验研究[J].中华医学杂志,2001,81(19):1194-1197.
[11]  Kouroumalis E, Skordilis P, Thermos K, et al. Treatment of hepatocellular carcinoma with octreotide: a randomised controlled study[J]. Gut, 1998,42:442-447.
[12]  Gulec SA, Drouant GJ, Fuselier J, et al. Antitumor and antiangiogenic effectors of somatostatin receptor-targeted in situ radiation with <111> In DTPAJ IC 2DL[J]. J Surg Res, 2001,97:131-137.
[13]  Buchan AM, Lin CY, Choi J, et al. Somatostatin, acting at receptor subtype 1,inhibits rho activity, the assembly of actin stress fibers, and cell migration[J]. J Biol Chem, 2002, 277:28431?28438.

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