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K-ras基因突变和Pax2、P53、Ki-67蛋白 在卵巢浆液性癌中表达的研究

Keywords: 卵巢浆液性癌,K-ras,Pax2,PCR

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Abstract:

目的研究低、高级别卵巢浆液性癌中K-ras基因第一外显子中第12位和第13位密码子点突变及Pax2、P53、Ki-67蛋白的表达。方法依照两级分级系统,分别选取低级别卵巢浆液性癌25例,高级别卵巢浆液性癌38例,另选取卵巢浆液性囊腺瘤20例作为对照组。应用PCR扩增K-ras基因目的片段并测序,免疫组织化学检测癌组织中的Pax2、P53、Ki-67蛋白的表达水平。结果卵巢浆液性癌中低级别组中K-ras突变率(28.6%)显著高于高级别组(5.3%),P<0.05,浆液性囊腺瘤中未检出突变。P53强阳性率在低、高级别浆液性癌中的表达率分别为28.0%和63.2%(P<0.05),在浆液性囊腺瘤中未表达。Pax2蛋白在低、高级别分别为48.0%和18.4%(P<0.05),在浆液性囊腺瘤中表达率为30%,与卵巢浆液性癌组差异无统计学意义。Ki-67蛋白在低、高级别组中的阳性率分别为16%和60.5%(P<0.05),在浆液性囊腺瘤中未表达。结论卵巢浆液性癌中K-ras基因突变主要发生在低级别浆液性癌中。P53、Pax2在低、高级别浆液性癌中表达差异有统计学意义,提示其在指导临床治疗中可能具有重要价值。

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